Micropatterned surfaces of PDMS as growth templates for HEK 293 cells |
| |
Authors: | R M Johann Ch Baiotto Ph Renaud |
| |
Institution: | (1) Fraunhofer-Institute for Biomedical Engineering, IBMT, D-66386 St. Ingbert, Germany;(2) Silicon Biosystems, I-40138 Bologna, Italy;(3) Swiss Federal Institute of Technology, EPFL, STI-LMIS, CH-1015 Lausanne, Switzerland |
| |
Abstract: | In this paper the easy and reliable preparation of precise micropatterns on PDMS surfaces is described and the growth of HEK
293 cells on those patterns during culture over several days is examined. The first patterning approach described is based
on soft-lithography and polyelectrolyte multilayer deposition. Two different soft-lithographic techniques are employed for
creating surface patterns of PAH, PSS, untreated and oxidized PDMS. The growth behavior of HEK 293 cells is investigated on
all the dual combinations of the four surfaces, and decreasing preference of the cells for the surfaces in the order PAH (–NH2) > ox-PDMS (–OH) >> PSS (–SO3
−) > PDMS (–CH3) is revealed. As the second patterning approach a method is introduced, which allows the deposition of gel droplets in a
microarray format utilizing differences in the surface wettability. This concept is new and expected to be very useful for
various applications. Finally, a speculative explanation for the different cell spreading behavior is provided considering
the interplay between individual cell–surface interactions and a permanent cell tractional force. |
| |
Keywords: | Surface micro patterning Polyelectrolyte multilayer Alginate hydrogel Cell adhesion Cell spreading Tissue engineering |
本文献已被 PubMed SpringerLink 等数据库收录! |
|