核因子-κappaB与肿瘤坏死因子α mRNA在肝细胞癌中的表达及其意义

目的：探讨核因子-κappaB(nuclear factor-κB,NF-κB)与肿瘤坏死因子α(tumor necrosis factor-alpha,TNF-α)mRNA在肝细胞癌中表达的意义。 方法：采用化学发光凝胶电泳迁移率(electrophoretic mobility shift assay,EMSA)的方法检测正常肝组织、肝血管瘤、肝癌癌灶及癌旁肝组织NF-κB的活性表达,用逆转录－聚合酶链反应(RT-PCR)方法检测TNF-α mRNA的表达。结果：正常肝组织、肝血管瘤TNF-α mRNA分别为0.24±0.12和0.21±0.10,显著低于癌旁肝组织和肝癌癌灶的0.36±0.16和0.68±0.21(P<0.05);正常肝组织、肝血管瘤NF-κB未被检测到明显的活性，而癌旁肝组织、肝癌癌灶NF-κB表达与正常肝组织及肝血管瘤组织比较差异具有显著性 (P<0.05)。癌旁肝组织、肝癌癌灶TNF-α mRNA表达与NF-κB的活性呈显著正相关(r=0.773,P<0.05; r=0.838,P<0.05)。 结论：NF-κB信号传导途径异常激活及TNF-α与肝细胞癌的发生发展密切相关。

Activation of nuclear factor-κB and expression of tumor necrosis factor-α mRNA in hepatocellular carcinoma

Abstract:Objective：To investigate the significance of activation of nuclear factor-κB(NF-κB) and expression of tumor necrosis factor-alpha(TNF-α)mRNA in hepatocellular carcinoma.Methods ：Chemoluminescent electrophoretic mobility shift assay(EMSA) was used to detect the activity of NF-κB in normal liver tissues, hemangiomas of liver, HCC tissues and tissues adjacent to cancer; and expression of TNF-α mRNA in each group was detected by RT-PCR analysis.Results：Expression of TNF-α mRNA in tissues adjacent to cancer and HCC tissues groups was 0.36±0.16 and 0.68±0.21,respectively, significantly higher than that in normal liver tissues and hemangiomas of liver 0.24±0.12 and 0.21±0.10 (all P<0.05). The activity of NF-κB in tissues adjacent to cancer and HCC tissues was significantly higher than that in normal liver tissues and hemangiomas of liver (P<0.05),and the expression of TNF-α mRNA in tissues adjacent to cancer and HCC tissues had a significant positive correlation with the activity of NF-κB(r=0.773,P<0.05; r=0.838,P<0.05, respectively).Conclusions：Abnormal activation of NF-κB signal transduction pathway and TNF-α was related to development and progression of HCC.