基于指纹图谱和多指标定量测定的苗药红禾麻质量控制研究

目的建立合理的苗药红禾麻Laporteabulbifera质量控制方法。方法采用高效液相色谱建立以指纹图谱为特征的多指标定性定量控制方法,对红禾麻12批次的药材进行质量控制,运用指纹图谱相似度评价系统对所测定的指纹图谱进行评价。结果建立了同时测定红禾麻药材中10个成分(新绿原酸、绿原酸、隐绿原酸、芦丁、异槲皮苷、木犀草苷、山柰酚-3-O-芸香糖苷、槲皮苷、表没食子儿茶素、表儿茶素)的多指标质量控制方法,该方法的线性关系、精密度、重复性和稳定性良好,加样回收率为95.89%～98.62%、RSD3%。同时测定了12批次红禾麻药材的指纹图谱并建立了共有模式,标定共有峰20个,12批次红禾麻药材指纹图谱相似度为0.805～0.931。结论所建立的基于指纹图谱和多指标含量测定的红禾麻质量控制方法,灵敏度高、准确度好、稳定可靠,为控制和评价红禾麻药材的内在质量提供科学依据。

Study on quality control of Miao medicine Laportea bulbifera based on fingerprint analysis and quantitative analysis of multi-components

Objective To establish a rational method for Laportea bulbifera quality control. Methods The fingerprint technique and multi-component quantitation were used to study the quality control of L. bulbifera by UHPLC. The 12 batches of L. bulbifera UHPLC fingerprint were evaluated by the evaluation system on similitude degree of chromatogram fingerprint of traditional Chinese medicine. Results The quality control methods of Miao medicine L. bulbifera for simultaneous determination of 10 components (including neochlorogenic acid, cryptochlorogenic acid, chlorogenic acid, rutin, isoquercitrin, luteoloside, kaempferol-3-O-rutinoside, quercitrin, epigallocatechin and epicatechin) were established. The linear, precision, repeatability and stability are good. The standard recoveries were 95.89%-98.62%, with RSD less than 3%. The common mode of fingerprint was established after determination fingerprints of 12 batches of samples of L. bulbifera by UHPLC. There were 20 common peaks in these samples. The similarity of the 12 batches fingerprints were in the range from 0.805 to 0.931. Conclusion The fingerprinting and multi-index content determination methods for quantitative control of Miao medicine L. bulbifera have high sensitivity, good accuracy, stability and reliability, which can provide a theoretical and experimental foundation for quantitative control of Miao medicine L. bulbifera.