HPLC-UV法测定大鼠血中[6]-姜酚的浓度

目的建立测定大鼠血浆中6]-姜酚浓度的HPLC-UV法,为进一步研究6]-姜酚的药代动力学提供可靠的方法。方法大鼠血浆样品以液-液萃取法提取后,采用HPLC-UV测定大鼠血浆中6]-姜酚的浓度。HPLC条件:Hypersil C18柱(250mm×4.6mm,5μm),乙腈-水(45:55)为流动相,进样量:20μL,流速:1.0mL/min,紫外检测波长:280nm。结果6]-姜酚线性范围为0.25～5.0μg/mL,最低检测限为0.1μg/mL,日内精密度RSD5.5%,日间精密度RSD6.8%。结论该测定方法具有灵敏、专一和快速的优点,可用于测定大鼠灌胃给药后6]-姜酚的血浆浓度。

Determination of [6]-Gingerol in Rat Plasma by HPLC-UV

Objective To develop a HPLC-UV method for the determination of 6]-gingerol in the rat plasma. Methods 6]-Gingerol was extracted from the rat plasma by using liquid-liquid extraction, then was separated on a Hypersil~(R) C_(18) column (250 mm×4.6 mm, 5 μm). The mobile phase was acetonitrile and water (45 : 55, v/v) with a flow-rate of 1.0 mL/min. The eluate from the HPLC column was monitored by the spectrophotometric detector at 280 nm. The injection volume was 20 μL. 6]-Gingerol was identified based on its retention time compared with the reference standard. Results The linear calibration curve was obtained in the concentration range of 0.25 ～5.0 μg/mL. The low quantitative limit was 0.1 μg/mL. The RSD of inter-day was < 5.5 % and that of intra-day precision was < 6.8 %. Conclusion The method is sensitive, specific and rapid to determinate the concentration of 6]-gingerol in the rat plasma after an oral administration.