毛细管电泳法测定白花丹参不同部位水溶性有效成分

目的:建立以高效毛细管区带电泳法快速分离和测定白花丹参不同部位水溶性有效成分的新体系。方法:采用未涂层石英毛细管柱(75μm×50.2cm,有效长度为40cm),以5mmol·L-1磷酸-硼砂缓冲液(pH7.4)为介质,压力进样(0.5psi)4s,20kV恒压分离,检测波长为210nm,柱温为25℃。结果:8min内白花丹参样品中的原儿茶醛、丹参素、原儿茶酸完全基线分离;三者的检测浓度均在2.5～200.0μg·mL-1范围内与各自峰面积积分值呈良好的线性关系;三者平均回收率分别为100.04%、99.99%、100.01%,RSD分别为1.75%、1.73%、1.74%。结论:本方法具有良好的精密度、回收率及线性关系,可用于定量测定白花丹参中3种水溶性有效成分。

Determination of Water-soluble Active Principles in Different Parts of Salvia miltiorrhiza by Capillary Zone Electrophoresis

OBJECTIVE：To establish a high performenc capillary zone electrophoresis method for the determination of the water- soluble active ingredients in different parts of the Salvia miltiorrhiza .METHODS： Capillary electrophoresis was conducted using uncoated capillary column（75 μm × 50.2 cm, effective length = 40 cm） with 5 mmol · L^- 1 phosphate-borax （ph7.4） as running buffer by pressure injection（0.5 psi/4 s）and constant voltage separation（20 kV） with a detection length of 210 nm and a column temperature of 25 12 .RESULTS： A complete baseline separation of PAH, DSS and PA was achieved within 8 min under the optimized conditions .The good linear range was from 2.5μg · mL^- 1 to 200.0μg · mL^- 1 for all the three ingredients.The average recoveries of the 3 ingredients were 100.04%, 99.99%, and 100.01%, respectively, with RSD at 1.75%, 1.73%, and 1.74%, respectively .CONCLUSION：The method is satisfactory in precision, recovery and linearity, and it can be used to determine three water-soluble active ingredients in different parts of the Salvia miltiorrhiza.