HBsAg阳性血清HBV DNA与HBV标志物定量测定的相关性特征

目的分析HBsAg阳性血清HBV DNA与HBV标志物（HBV M）含量之间的量化关系,揭示两种HBVM血清学模式HBV DNA与HBV M含量的相关性特征。方法对225例血清标本采用PE7000型FQ-PCR仪和AXSYM免疫分析仪分别进行HBV DNA和HBVM的定量测定,以HBV M血清学模式进行HBV DNA分组并分析比较各组的相关性及特点。结果HBsAg/HBeAg/抗-HBc阳性模式的HBV DNA含量54.5%分布在107拷贝/ml以上,HBV DNA的C~G组及抗-HBs、HBeAg、抗-HBe的F~G组含量分别与A组比较差异有统计学意义,且HBV DNA含量与抗-HBs呈负相关（r=-0.670）、与HBeAg和抗-HBe则呈正相关（r=0.524、r=0.814）;而HB-sAg/抗-HBe/抗-HBc阳性模式的HBV DNA含量82.2%分布在105拷贝/ml以下,HBV DNA的B~G组分别与A组比较差异有统计学意义,且仅与抗-HBs呈负相关（r=-0.569）。结论不同HBV M血清学模式HBV DNA与HBV M含量之间存在不同的相关性特征。

Correlation of quantitative determination of HBV DNA and HBV markers in HBsAg positive sera

Objective To reveal the correlation of quantitative determination of HBV DNA and HBV markers in HBsAg positive sera.Methods HBV DNA and five HBV markers (including HBsAg, anti-HBs, HBeAg, anti-HBe and anti-HBc) in 225 samples of HBsAg positive sera were quantitatively determined by using PE7000 FQ-PCR analyser and AXSYM instrument respectively.Results The HBV DNA level in 54.5% of samples with HBsAg/HBeAg/anti-HBc positive was above 107copies/ml, and that of 82.2% of samples with HBsAg/anti-HBe/anti-HBc was below 105copies/ml.As compared with that of A group, the differences was significantly in C~G groups and B~G groups of HBV DNA, F~G groups of anti-HBs, HBeAg, anti-HBe. In F~G groups, the correlation coefficient was -0.670 of HBV DNA and anti-HBs, 0.524 of HBV DNA and HBeAg, 0.814 of HBV DNA and anti-HBe. In B~G groups, the correlation coefficient was -0.569 of HBV DNA and anti-HBs.Conclusion The relativity characterization is different between HBV DNA and HBV M in different serological patterns.