| 兔骨髓间充质干细胞体外培养定向诱导分化为软骨细胞 |
| |
| 引用本文： | 俞猛,于方,付胜良.兔骨髓间充质干细胞体外培养定向诱导分化为软骨细胞[J].中国组织工程研究与临床康复,2011,15(27). |
| |
| 作者姓名： | 俞猛 于方 付胜良 |
| |
| 作者单位： | 首都医科大学附属北京世纪坛医院骨科,北京市,100038 |
| |
| 摘 要： | 背景:骨髓间充质干细胞具有分化为骨、软骨、肌腱、脂肪等组织的多分化潜能.目的:体外培养兔骨髓间充质干细胞并定向诱导分化为软骨细胞,探讨体外诱导分化为软骨的方法和条件.方法:取兔股骨骨髓,全骨髓贴壁法分离培养骨髓间充质干细胞,碱性成纤维生长因子体外培养后,取第3 代细胞在培养基中添加不同质量浓度转化生长因子β1 的软骨分化诱导剂,2 周后倒置显微镜观察细胞形态,甲苯胺蓝染色,RT-PCR技术检测Ⅱ型胶原和聚集蛋白聚糖的表达.结果与结论:可以从兔骨髓中培养出骨髓间充质干细胞,碱性成纤维生长因子能明显的促进骨髓间充质干细胞增殖.经软骨诱导分化后骨髓间充质干细胞呈软骨细胞形态,甲苯胺蓝染色阳性,Ⅱ型胶原和聚集蛋白聚糖的表达阳性.10 μg/L 的转化生长因子β1 诱导成软骨分化能力最强.
|
| 关 键 词： | 转化生长因子β1 骨髓间充质干细胞 碱性成纤维生长因子 软骨分化 兔 |
Differentiation of rabbit mesenchymal stem cells into chondrocytes in vitro |
| |
| Yu Meng,Yu Fang,Fu Sheng-liang.Differentiation of rabbit mesenchymal stem cells into chondrocytes in vitro[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(27). |
| |
| Authors: | Yu Meng Yu Fang Fu Sheng-liang |
| |
| Institution: | Yu Meng,Yu Fang,Fu Sheng-liang Department of Orthopedics,Beijing Shijitan Hospital Affiliated to Capital Medical University,Beijing 100038,China |
| |
| Abstract: | BACKGROUND:Bone marrow mesenchymal stem cells(BMSCs) have a multi-differentiated potential to differentiate into bone,cartilage,tendon,adipose and so on.OBJECTIVE:To build a method of differentiation of rabbit BMSCs into chondrocytes in vitro and to determine the factors involved in the differentiation process.METHODS:BMSCs were isolated from young rabbit femur marrow,and then planted in plastic culture flask.After stimulated by basic fibroblast growth factor(bFGF),passage 3 cells were chosen to induce into... |
| |
| Keywords: | |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
