| 冻干韧带脱细胞支架材料的生物相容性及优势 |
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| 引用本文： | 王辉,陈雄生,周盛源,黄俊俊,蔡弢艺.冻干韧带脱细胞支架材料的生物相容性及优势[J].中国组织工程研究与临床康复,2011,15(29). |
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| 作者姓名： | 王辉 陈雄生 周盛源 黄俊俊 蔡弢艺 |
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| 作者单位： | 解放军第二军医大学附属长征医院骨科,上海市,200070 |
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| 基金项目： | 国家高技术研究发展计划(863 计划)项目，课题名称:转基因骨髓间充质干细胞与脱细胞支架构建组织工程韧带的实验研究 |
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| 摘 要： | 背景:韧带脱细胞基质是通过各种脱细胞方法将韧带组织内的细胞成分清除,降低免疫原性,同时对纤维支架结构破坏轻微,保留了细胞外基质的机械性能.目的:评价冻干兔髌韧带脱细胞支架的生物相容性及优势.方法:取兔髌韧带,利用1%脱氧胆酸钠行脱细胞处理,制备冻干和未冻干脱细胞韧带支架.结果与结论:冻干韧带脱细胞支架保持了冻干前的胶原结构与力学特征,无细胞残留,其浸提液对细胞生长无抑制作用,无全身急性毒性反应,无热原存在,且原发刺激指数为0分,皮内刺激实验阴性.体内埋植实验显示冻干韧带脱细胞支架表现为免疫原性小,炎症反应轻的特点.说明冻干韧带脱细胞支架具有较好的生物相容性,且制作简单,便于消毒、包装、保存.
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| 关 键 词： | 组织工程 韧带脱细胞支架 韧带 脱细胞 冻干 生物相容性 |
Biocompatibility and superiority of lyophilized acellular ligament scaffolds |
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| Wang Hui,Chen Xiong-sheng,Zhou Sheng-yuan,Huang Jun-jun,Cai Tao-yi.Biocompatibility and superiority of lyophilized acellular ligament scaffolds[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(29). |
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| Authors: | Wang Hui Chen Xiong-sheng Zhou Sheng-yuan Huang Jun-jun Cai Tao-yi |
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| Institution: | Wang Hui,Chen Xiong-sheng,Zhou Sheng-yuan,Huang Jun-jun,Cai Tao-yi Department of Orthopedics,Changzhen Hospital,Second Military Medical Univeristy of Chinese PLA,Shanghai 200070,China |
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| Abstract: | BACKGROUND: Acellular matrix ligament removes the cellular components within the ligament tissue and reduce the immunogenicity through a variety of acellular ways. Simultaneously, the damage to scaffold structure is mild in the process of decellularization, and it retains the mechanical properties of the extracellular matrix. OBJECTIVE: To verify the biocompatibility and superiority of rabbit patellar tendon acellular scaffold after frozen and lyophilized processing. METHODS: Patellar ligaments were treated... |
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