| 介绍一种简便、快速、大量制备人血清载脂蛋白AⅠ、AⅡ及其抗血清的方法 |
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| 引用本文： | 傅阳恒,杨秀芳,何德华,朱禧星,钟学礼. 介绍一种简便、快速、大量制备人血清载脂蛋白AⅠ、AⅡ及其抗血清的方法[J]. 复旦学报(医学版), 1988, 0(3) |
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| 作者姓名： | 傅阳恒 杨秀芳 何德华 朱禧星 钟学礼 |
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| 作者单位： | 上海医科大学华山医院糖尿病研究室(傅阳恒，杨秀芳，何德华，朱禧星)，上海医科大学华山医院糖尿病研究室(钟学礼) |
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| 摘 要： | 采用80ml的PC管一次性超速离心分离入血清极低密度脂蛋白、低密度脂蛋白、高密度脂蛋白;用DEAE-52离子交换柱层析同时纯化载脂蛋白AI(ApoAl)、AII(ApoAII)。经SDS-聚丙烯酰胺凝胶电泳测定ApoAI分子量27000u(原子质量单位)、ApoAII18000u(原子质量单位)。经SDS-PAGE,双向免疫扩散及免疫电泳证实其为纯品。采用上述抗原免疫新西兰白兔制备的抗ApoAI、抗ApoAII血清经双向免疫扩散,免疫电泳证实为单价抗备清。这种分离提纯ApoAI、ApoAII的方法具有简便、快速、价廉、适于大量制备等优点。
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| 关 键 词： | 载脂蛋白AⅠ、AⅡ 分离与纯化 抗血清 |
A SIMPLE, RAPID METHOD FPR PREPARING APOLIPOPROTEIN AI,AII AND ANTIBODIES IN HUMAN SERUM |
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| Fn Yangheng,Yang Xiufang,He Dehua,Zhu Xixing,Zhong Xneli. A SIMPLE, RAPID METHOD FPR PREPARING APOLIPOPROTEIN AI,AII AND ANTIBODIES IN HUMAN SERUM[J]. Fudan University Journal of Medical Sciences, 1988, 0(3) |
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| Authors: | Fn Yangheng Yang Xiufang He Dehua Zhu Xixing Zhong Xneli |
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| Abstract: | An 80ml PC bottle was used to separate VLDL, LDL, and HDL in human serum simultaneously. Apolipoprotein AI, AII(ApoAI, AII) were purified by means of DEAE-52 ohromatography. Molecular weights of ApoAII and AII determined by SDS-polyaorylamide-gel eleotrophoresis were 27 000 and 18 000 respectively. Purified ApoAI and ApoAII were proved to be the pure proteins by SDS-PAGE, double immune diffusion, and immune electrophoresis. Anti-ApoAI, anti-ApoAII serum were prepared in New Zealand rabbits and were proved to be specific antibodies to ApoAI and ApoAII by doable immune diffusion and immune eleotrophoresis. The method of isolation and purification of ApoAI and ApoAII is a simple, rapid, comparatively inexpensive procedure and is applicable to mass production. |
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| Keywords: | apolipoprotein AI AII Isolation and purification anti-Serum |
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