基于网络药理学探讨祛湿通脉法在类风湿性关节炎中的作用

目的通过网络药理学探究全蝎、益母草、金钱草治疗类风湿性关节炎(rheumatoid arthritis,RA)的药理机制,为祛湿通脉法的临床运用提供理论依据。方法基于TCMSP、TCMID数据库搜集全蝎、益母草、金钱草成分及靶点,通过Cytoscape软件构建成分-靶点网络;通过TTD、DrugBank数据收集RA相关靶点,在STRING数据库构建靶点互作PPI网络。在Genomics数据库分析药物和疾病共有靶点,并利用Cytoscape软件中Cluego插件分析共同靶点的信号通路,最后构建成分-靶点-通路网络图并分析。结果益母草、金钱草、全蝎发挥治疗RA的成分可能是槲皮素、山柰酚等;发挥治疗作用的靶点可能包括IL-6、TNF、JUN、PTGS1、PTGS2、PIK3CG等;其关键作用通路包括L-17信号通路、肿瘤坏死因子信号通路、NF-κB信号通路等13条。结论益母草、金钱草、全蝎治疗RA作用的药理学机制可能是通过多组分、多靶点、多通路实现,祛湿通脉法运用于RA治疗具有一定的价值。

Roles of Dampness-removing and Pulse-dredging Methods in Treatment of Rheumatoid Arthritis Based on Network Pharmacology

Objective To explore the pharmacological mechanism of Buthus martensi,Leonuri HerbaandLysimachiae Herbaon rheumatoid arthritis(RA)through network pharmacologyandprovide theoretical basis for the clinical application of dampness-removing and pulse-dredging methods.Methods This study collected the components and targets of four herbs in TCMSP and TCMID databases and settled a compound-target protein network by Cytoscape software.The RA-related targets were collected from TTD and DrugBank which were constructed as PPI network inSTRING database.The targets of drugs and diseases were analyzed in Genomics database.The Cluego plug-in in Cytoscape software was used to analyze the signal pathway.Finally,the component-target-pathway network was constructedby Cytoscape software.Result:The compounds that playroles intreatment of RA might be quercetin,kaempferol,etc.The targets that are important in RA might be IL-6,TNF,PTGS1,PTGS2,etc.The key pathways include IL-17 signaling pathway,TNF signaling pathway,NF-kappa B signaling pathway,etc.Conclusion:The pharmacological mechanism of herbs in treatment of RA may be based on multi-component,multi-target and multi-pathway.The application of dampness-removing and pulse-dredging methods inRA has apotential therapeutic value.