欣胃颗粒对胃癌前病变大鼠胃黏膜关键因子Akt、P-GP表达的影响

目的探讨欣胃颗粒对胃癌前病变(PLGC)大鼠胃黏膜Akt、P-gp表达的影响。方法将健康的雄性Wistar大鼠120只根据体质量编号,按随机数字表法将Wistar大鼠随机分为4组。除空白组外,其他3组均自由饮用N-甲基-N’-硝基-N-亚硝基胍(MNNG),采用标准的饲料雷尼替丁喂养,热盐水灌胃,同时配合饥饱失常的复合造模法进行造模。至16周结束,随机抽取模型组大鼠4只做胃黏膜病理组织学检查,确认模型成功后全部停止造模及干预药物。模型制备成功后随即处死所有大鼠,剖腹取胃,应用免疫组织化学法、Western Blot法检测胃黏膜组织中Akt、P-gp基因和蛋白的表达情况。结果模型组胃黏膜Akt、P-gp的表达较其他各组显著升高,欣胃颗粒可降低Akt、P-gp基因和蛋白的表达。结论欣胃颗粒可通过下调PLGC大鼠模型胃组织Akt、P-gp基因和蛋白的表达,从而促进抗细胞的增殖。

Effect of Xinwei Granules on Expressions of Akt and P-gpof Gastric Mucosa in Rats with Gastric Precancerous Lesion(PLGC)

Objective To investigate the effect of Xinwei Granules on the expressions of Akt and P-gp in gastric mucosa of rats with gastric precancerous lesion(PLGC). Methods A total of 120 healthy male Wistar rats were numbered by weight and divided into 4 groups according to by random number table method. Except for the blank group, rats of the other three groups were administered with N-methyl-N’-nitro-N-nitrosoguanidine(MNNG) for drinking freely, fed with standardranitidine, and gavaged with heat saline, combining with the composite modeling method characterized with abnormal of starvaion.At the end of the 16 th week, 4 rats in the model group were randomly selected for histopathologic examination of gastric mucosa, and intervention drugs were stopped after the success of the model.All rats were killed immediately after the model was successfully prepared. We took out the stomachand used immunohistochemistry and Western blot method to detect the gene expressions of Akt and P-gp and protein in gastric mucosa. Results The expressions of Akt and P-gp in gastric mucosa of model group was significantly higher than that of other groups. Xinwei Granules could decrease the gene and protein expressions of Akt and P-gp.Conclusion Xinwei Granules can facilitate gastric epithelial cell apoptosis and inhibit the proliferation by down-regulating the gene and protein expressions of Akt and P-gp in gastric tissues of PLGC rats.