分枝杆菌细菌学检测方法的临床评价

目的评价常用分枝杆菌细菌学检测方法对住院结核病人诊断的临床效果。方法对1147例住院结核病患者采用涂片镜检、罗氏培养、MB/BacT液体培养和PCR扩增等四种方法检测的阳性检出率和阳性检测时间进行比较。结果四种方法对不同临床标本的阳性检出率分别为14.2%、23.7%、22.3%和15.9%,阳性检测时间分别为1天、23.1天、18.8天和2.8天。涂片镜检与其他三种方法联合使用可以分别使菌阳检出率提高28.6%、29.2%和27.3%。结论常规方法在阳性检出率和阳性检测时间方面各具优势,合理配合使用可以明显提高临床结核病细菌学诊断的敏感性和便捷性。

Comparison of the Conventional Diagnostic Modalifies, MB/BacT Culture and PCR Test for Diagnosis of Tuberculosis

Objective To evaluate the performance of M.tuberculosis examination in clinical samples obtained from pulmonary and extrapulmonary cases of tuberculosis.Methods Samples of 1147 clinical TB patients were processed for detection of Mycobacterium tuberculosis by ZN smear examination,LJ medium culture,MB/BacT culture and PCR tests.Results Significant differences were seen in the sensitivities of different tests,they were 14.2% for ZN smear examination,23.7% for LJ culture,22.3% for MB/BacT culture,and 15.9% for PCR test.When ZN smear examination combined with other three methods.their positive rates were be increased by 28.6%,29.2%,and 27.3% respectively The mean detection time for M.tuberculosis was 23.1 days by LJ medium culture,18.8 days by MB/BacT culture and 2.8 days by PCR test.Conclusion All the four methods have their own advantages,combined application of then might be an effective measurement in clinical diagnosis of TB.