复合吸收促进剂对葛根素肠吸收及毒性的影响

目的 考察吸收促进剂配伍对葛根素肠吸收的影响,并进一步观察复合吸收促进剂对肠黏膜的毒性.方法 运用外翻肠囊法,采用L9(3)4正交设计试验考察各吸收促进剂的协同作用,确定较理想复合吸收促进剂;运用显微形态学观察复合吸收促进剂对肠黏膜的毒性.结果 复合吸收促进剂:1%壳聚糖、0.02%白芷挥发油、0.06%冰片配伍具有协同作用,可显著提高葛根素的肠吸收;复合吸收促进剂无肠黏膜毒性.结论 1%壳聚糖、0.02%白芷挥发油、0.06%冰片配伍可安全有效地促进葛根素的肠吸收.

Effect of multi-combination absorption enhancer on puerarin intestinal absorption and its toxicity

Objective To investigate the influence of the compatibility of absorption enhancers (Aes) on the intestinal absorption of puerarin and study the Aes' toxicities to the intestinal. Methods The in vitro everted intestinal sac was used as model. An orthogonal L9 (3)4 test design was used to optimize a best multi-combination of Aes and investigate the synergistic effect of the optimized Aes on intestinal per-meability of puerarin. And the Aes' toxicities were further studied by light microscopy. Results The re-sults showed that a multi-combination which including 1% chitosan, 0. 02% oil from Radix Angelicae, and 0.06% borneol had synergistic effect, which could significantly enhance the intestinal absorption of puerarin. And the multi-combination had no toxieities. Conclusion A multi-combination which ineluding 1 % chitosan, 0.02 % oil from Radix Angelicae Dahuricae, and 0.06 % borneol could safely and efficiently enhance the intestinal absorption of puerarin.