青藤碱对大鼠肾移植急性排斥反应的抑制作用

目的探讨青藤碱免疫抑制作用,为其在器官移植免疫抑制治疗上的应用提供实验依据.方法建立48个大鼠原位肾移植急性排斥反应模型(Wistar-SD)分为四组:分别于腹腔注射生理盐水(1 ml/(kg.d))、青藤碱(30 mg/(dg.d))、环孢菌素A(2.5 mg/(kg.d))、青藤碱(30mg/(kg.d)) 环孢菌素A(2.5 mg/(kg.d)),每组12例,随机抽取6例观察受者存活时间,另6例于术后第六天处死,取腔静脉血测尿素氮及肌酐,取移植肾组织标本病检.分析青藤碱协同环孢菌素A对大鼠肾移植受者存活时间、肾功能指标BUN、Cr以及对移植肾组织病理变化的影响.结果青藤碱[30mg/(kg·d)]可延长受体鼠存活时间至(9.67±0.52)d,与环孢菌素A[2.5mg/(kg·d)]联用后可明显延长受体鼠存活时间至18天以上(25.00±4.65)d;青藤碱对移植肾的功能表现出保护作用并改善移植肾组织的病理变化.结论青藤碱对大鼠肾移植的急性排斥反应起到一定的抑制作用,并与亚治疗剂量的环孢菌素A产生协同作用,青藤碱是一种从自然药物中提取的具有开发前景的免疫抑制药物.

Inhibitory effect of Sinomenine on acute rejection reaction in renal transplantation of rat

Objective:To study immune inhibitory effect of Sinomenine(SIN)and provide experimental gist for application of SIN in organic transplantation.Methods:48 acute rejection reaction models(Wistar-SD)of orthotopic renal transplantation of rat were established and randomly divided into 4 groups(12 rats each group):respectively abdominal injection of 0.9% sodium chloride solution(1 ml/(kg.d));Sinomenine(SIN)(30lected from each group were killed on the postoperative sixth day(acute rejection reaction stage),sampling of blood from inferior vena cava for test of creatinine(Cr)and urea nitrogen(BUN)in blood.Tissue of transplanted kidney was gotten for pathological test.Affection of pathological changes of SIN combined with CsA on survival time of renal recipient,kidney function index BUN,Cr in renal transplantation of rat and pathological changes of transplanted kidney were analyzed.Results:SIN(30 mg/(kg.d))might prolong survival time of recipient rat to(9.67+0.52)day,combined application of CsA(2.5 mg/(kg-d)might markedly prolong survival time of recipient rat to over 18 day(25.00±4.65);SIN has definite inhibition effect on acute rejection reaction in renal transplantation,and a cooperation effect with sub-treatment dosage of Cyclosporin A(CsA).SIN is a natural drug with good explitation future in immune inhibition.