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20种中药水溶性糖HPLC指纹图谱与寒热药性关系研究
引用本文:李静文,李峰,周正礼. 20种中药水溶性糖HPLC指纹图谱与寒热药性关系研究[J]. 山东中医药大学学报, 2010, 0(3)
作者姓名:李静文  李峰  周正礼
作者单位:山东中医药大学药学院;
基金项目:国家重点基础研究发展计划(“973”计划)项目(编号:2007CB512601); 山东省科技发展计划课题(编号:2006GG2202017、2006GG3202003)
摘    要:
目的:探讨中药寒热药性与水溶性糖的相关性,进一步阐明中药四性与其物质基础的关系。方法:选取10种寒性中药和10种热性中药,提取水溶性糖,加入1-苯基-3-甲基-5-吡唑啉酮进行衍生化反应,测定水溶性糖的HPLC指纹图谱,利用Fisher方法建立判别函数,分析实验结果。结果:建立寒热药性的Fisher判别函数,实现了对20种中药寒热药性的准确判别,判别正确率为100%。结论:中药寒热药性与水溶性糖存在明显的相关性,Fisher可以作为判别中药寒热药性与水溶性糖关系的统计学工具,水溶性糖是中药寒热药性的物质基础之一。

关 键 词:寒热药性  水溶性糖  HPLC  指纹图谱  Fisher分析

Relationship between high performance liquid chromatographic fingerprints of water-soluble sugar of 20 kinds of traditional Chinese herbs and both cold and heat properties
LI Jing-wen,LI Feng,ZHOU Zheng-li. Relationship between high performance liquid chromatographic fingerprints of water-soluble sugar of 20 kinds of traditional Chinese herbs and both cold and heat properties[J]. Journal of Shandong University of Traditional Chinese Medicine, 2010, 0(3)
Authors:LI Jing-wen  LI Feng  ZHOU Zheng-li
Affiliation:LI Jing-wen,LI Feng,ZHOU Zheng-li(Chinese Materia Medica Faculty,Sh,ong University of Traditional Chinese Medicine,Jinan 250355,China)
Abstract:
Objective:To discuss the correlation between both cold and heat properties and water-soluble sugar,and reveal the relation of four properties and their material basis.Methods:Extracting water-soluble sugar respectively from 10 kinds of cold herbs and heat herbs,1-phenyl-3-methyl-5-pyrazolone were added to the liquid for derivative reaction.The HPLC fingerprints of water-soluble sugar were analyzed by Fisher method through establishing discriminant function.Results:Fisher dicriminant function was established...
Keywords:cold-heat properties  water-soluble sugar  high performance liquid chromatography  fingerprints  Fisher analysis  
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