无肝状态下丙泊酚代谢相关UGT1A6在大鼠肝外器官的基因表达

目的:研究无肝期前、后与丙泊酚代谢相关的代谢酶UGT1A6在大鼠小肠、肾、肺、脑器官组织中基因表达的变化,以期初步阐释丙泊酚无肝期肝外代谢特点形成的原因。方法:15只大鼠单次注射丙泊酚后随机分为3组(n=5),A组为对照组,B组阻断肝门30min,C组阻断肝门60min,截取各组大鼠小肠、肾、肺、脑器官组织,采用逆转录聚合酶链式反应(RT-PCR)技术,检测各组UGT1A6mRNA的表达。结果:大鼠各受检器官组织中UGT1A6的基因表达水平B、C组明显高于A组(P<0.01);B组与C组相比,没有显著性差异(P>0.05)。结论:大鼠受检组织中UGT1A6基因表达在无肝期比无肝期前增多,系可能促进丙泊酚无肝期肝外代谢增强的原因。

Expression of Extrahepatic Gene of Propofol Metabolism-Related UGT1A6 in Anhepatic Phase

OBJECTIVE：To study the variation of gene expression of propofol metabolism- related UGT1A6 in small intestine, kindney, lung and brain of rats before and after anhepatic phase so as to reveal the mechanism of extrahepatic metabolism characters of propofol. METHODS： 15 rats were divided into 3 groups after injection of single dose of propofol （n = 5） ： group A （control group）, group B in which the hepatic portal was devascularized for 30min, and group C in which the hepatic portal was devascularized for 60 min. Small intestine, kindney, lung and brain tissues of rats were taken for the detection of UGT1A6 mRNA by RT - PCR techniques. RESULTS： Gene expression levels of UGT1A6 in small intestine, kindney, lung and brain in group B and group C were significantly higher than in group A（P〈0.01）; The gene expression levels between B and C had no significant difference （P 〉0.05）. CONCLUSION： Gene expression levels of extrahepatic UGT1A6 of rats are increased in anhepatic phase as compared with before anhepatic phase, which might be attributed to the enhancement of extrahepatic metabolism of propofol in anhepatic phase.