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| Analysis of the Conservation of T Cell Receptor Alpha and Beta Chain Variable Regions Gene in pp65 Peptide-Specific HLA-A＊0201-Restricted CD8^＋ T Cells | |
| 引用本文： | Wei Luo,Li Ma,Qian Wen,Mingqian Zhou,Xiaoning Wang. Analysis of the Conservation of T Cell Receptor Alpha and Beta Chain Variable Regions Gene in pp65 Peptide-Specific HLA-A＊0201-Restricted CD8^＋ T Cells[J]. Cellular & molecular immunology, 2009, 6(2): 105-110. DOI: 10.1038/cmi.2009.14 |
| 作者姓名： | Wei Luo Li Ma Qian Wen Mingqian Zhou Xiaoning Wang |
| 作者单位： | Wei Luo,Qian Wen,Mingqian Zhou(Institute of Molecular Immunology, School of Biotechnology, Southern Medical University, Guangzhou 510515, China);Li Ma(Institute of Molecular Immunology, School of Biotechnology, Southern Medical University, Guangzhou 510515, China;Institute of Molecular Immunology, School of Biotechnology, Southern Medical University, Guangzhou 510515, China);Xiaoning Wang(School of Biosciences & Bioengineering, South China University of Technology, Guangzhou 510641, China;School of Biosciences & Bioengineering, South China University of Technology, Guangzhou 510641, China) |
| 基金项目： | 国家自然科学基金，教育部新世纪优秀人才支持计划，广东省自然科学基金，国家重点基础研究发展规划(973计划) |
| 摘 要： | Many viral epitope specific T cell receptors （TCRs） in MHC-matched individuals have been demonstrated to involve conserved amino acid motifs in β chain complementarity-determining region 3 （CDR3）. However, it is not sure whether the conserved motifs can also be found in TCR β chain. In previous studies, we developed a modified method to enlarge the percentage of cytomegalovirus （CMV） pp65 peptide-specific CD8^＋ T cells in PBMC by continuous peptide stimulation in vitro, which provides sufficient number of specific T cells for detection. In this study, we further analyzed the restrictive usage of TCR Vα and Vβ gene families and investigated the CDR3 gene sequence of pp65 peptide-specific CD8β T cells. Analysis of CDR3 spectratypes suggested a restricted usage of TCR α chain AV8, AV12, AV21, AV31 families and TCR βchain BV3, BV14, BV21, BV23, BVll families in donor CD8^＋ T cells stimulated by pp65 peptide. The sequences of these T cells involved similar sequence （TX） G （X） A in CDR3 region of TCR α chain and L （XT） G （X） A in TCR β chain. |
| 关 键 词： | 人类白细胞抗原 T细胞受体 抗原特异性 pp65 多病毒 CD8 受体α 可变区基因 |
| 收稿时间： | 2009-01-12 |
Analysis of the Conservation of T Cell Receptor Alpha and Beta Chain Variable Regions Gene in pp65 Peptide-Specific HLA-A~*0201-Restricted CD8~+ T Cells |
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| Wei Luo,Li Ma,,Qian Wen,Mingqian Zhou , Xiaoning Wang. Analysis of the Conservation of T Cell Receptor Alpha and Beta Chain Variable Regions Gene in pp65 Peptide-Specific HLA-A~*0201-Restricted CD8~+ T Cells[J]. Cellular & molecular immunology, 2009, 6(2): 105-110. DOI: 10.1038/cmi.2009.14 | |
| Authors: | Wei Luo Li Ma Qian Wen Mingqian Zhou Xiaoning Wang |
| Affiliation: | Wei Luo1,Li Ma1,3,Qian Wen1,Mingqian Zhou1 , Xiaoning Wang2,31Institute of Molecular Immunology,School of Biotechnology,SouthernMedical University,Guangzhou 510515,China,2School of Biosciences & Bioengineering,South China University ofTechnology,Guangzhou 510641 |
| Abstract: | Many viral epitope specific T cell receptors (TCRs) in MHC-matched individuals have been demonstrated to involve conserved amino acid motifs in β chain complementarity-determining region 3 (CDR3). However, it is not sure whether the conserved motifs can also be found in TCR α chain. In previous studies, we developed a modified method to enlarge the percentage of cytomegalovirus (CMV) pp65 peptide-specific CD8+ T cells in PBMC by continuous peptide stimulation in vitro, which provides sufficient number of specific T cells for detection. In this study, we further analyzed the restrictive usage of TCR Vα and Vβ gene families and investigated the CDR3 gene sequence of pp65 peptide-specific CD8+ T cells. Analysis of CDR3 spectratypes suggested a restricted usage of TCR α chain AV8, AV12, AV21, AV31 families and TCR β chain BV3, BV14, BV21, BV23, BV11 families in donor CD8+ T cells stimulated by pp65 peptide. The sequences of these T cells involved similar sequence (TX) G (X) A in CDR3 region of TCR α chain and L (XT) G (X) A in TCR β chain. |
| Keywords: | TCR CDR3 CMV pp65 peptide CTL |
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