养肝利胆颗粒对胆囊胆固醇结石豚鼠胰岛素抵抗的干预作用

目的：观察养肝利胆颗粒对胆囊胆固醇结石豚鼠胰岛素抵抗的调节作用。方法：选用80只豚鼠，雌雄各半，随机分成正常对照组、模型组、中药治疗组和西药治疗组共4组。用高脂高胆固醇饲养法建立胆囊胆固醇结石豚鼠模型。正常对照组和模型组灌服生理盐水，中药治疗组灌服养肝利胆颗粒，西药组灌服熊去氧胆酸。造模第49天每组各取8只进行高胰岛素-正葡萄糖钳夹实验，于实验期间测定葡萄糖输注率。实验结束处死豚鼠取材，观察胆结石成石率。结果：经胆结石红外光谱定性分析，豚鼠胆囊结石均为胆固醇结石。模型组成石率为83．25％。高胰岛素-正葡萄糖钳夹实验中模型组豚鼠葡萄糖输注率较正常对照组明显降低。与模型组相比，养肝利胆组与熊去氧胆酸组葡萄糖输注率明显升高，且养肝利胆组升高更为明显。结论：养肝利胆颗粒可以明显升高葡萄糖输注率，改善胆囊胆固醇结石豚鼠的胰岛素抵抗程度。

Effects of Yanggan Lidan Granule on insulin resistance in guinea pigs with induced cholesterol gallstones

Objective： To observe the effects of Yanggan Lidan Granule （YGLDG）, a compound traditional Chinese herbal medicine, on insulin resistance in guinea pigs with induced cholesterol gallstones. Methods： Eighty guinea pigs were randomly divided into normal control group, untreated group, YGLDG group and ursodeoxycholic acid （UDCA） group, with 20 guinea pigs in each group. Except the normal control group, gallstones were induced by high-cholesterol diet in the guinea pigs. The guinea pigs in the normal control group and the untreated group were administered with normal saline. UDCA and YGLDG were given to the guinea pigs in the corresponding groups for seven weeks. Eight guinea pigs of each group were used to measure the glucose infusion rate （GIR） by using hyperinsulinemic-euglycemic clamp technique. At the end the guinea pigs were killed and their gallstone formation was observed. Results： The gallstones in guinea pigs were identified as cholesterol stones by qualitative analysis through infrared spectrum. The incidence rate of cholelithiasis of the untreated group was 82. 35% . The GIR of guinea pigs in the untreated group was obviously lowered down as compared with the normal control group. Compared with the untreated group, the GIRs of the YGLDG group and the UDCA group were obviously increased, especially in the YGLDG group. Conclusion： YGLDG may improve insulin resistance in guinea pigs with cholesterol gallstones by elevating GIR obviously.