低浓度砷暴露者皮肤损害及DNA氧化损伤

目的 探讨女性慢性低浓度砷(As)暴露者皮肤损害情况及与DNA氧化损伤的关系。方法 选择山西大同某村长期饮水型低As暴露女性54人(病例组)和经济水平相近的邻村女性18人(对照组)作为研究对象,测定当地水As及研究对象体内尿As水平;并测定尿中8-羟基脱氧鸟苷(8-OHdG)水平。结果 低As暴露组人群体内尿As平均水平为(13.99±4.65)μg/gCr,尿8-OhdG平均水平为(8.61±4.51)ng/mgCr,均高于对照组(P<0.05);尿中8-OHdG与尿As水平呈正相关(r=0.893,P<0.05);尿中8-OHdG水平与水As暴露程度呈正相关(r=0.847,P<0.05);尿8-OHdG水平较高者发生皮肤损害的危险性是较低者的2.838倍(OR=2.838,P<0.05)。结论 低浓度砷As暴露可致机体产生DNA氧化损伤;尿8-OHdG可作为一个稳定的As致氧化损伤生物标志物。

Correlation between skin lesion and DNA oxidative damage in women exposed to low concentration arsenic

Objective To explore the correlation between skin lesion and DNA oxidative damage in the women exposed to low concentration arsenic.Methods Fifty-four female cases exposed to arsenic in drinking water and 18 females controls were investigated.The urine samples were collected from the females and measured for urinary total arsenic and 8-hydroxy-2'-deoxyguanosine(8-OHdG).Results The average total urinary arsenic was 22.90±6.41 μg/gCr and the average contert of 8-OHdG was 11.78±2.95 μg/gCr for the chronic arsenic exposure,both of them were significantly higher than those of the control.Significant positive correlations were found between urinary 8-OHdG and total arsenic(r=0.893,P<0.05)as well as urinary 8-OHdG and the grade of skin lesion(r=0.847,P<0.05).The level of urinary 8-OHdG was associated with an increased risk of skin lesion in arsenic exposed cases(odds ratio=2.838,P<0.05).Conclusion A clear dose-response relationship was observed between low arsenic exposure and skin lesion.The results suggest low arsenic exposure could lead to oxidative DNA damage and 8-OHdG can be used as a stable biomarker for arsenic-induced oxidative damage.