| 高速逆流色谱法从棉花花提取物中分离制备异槲皮素 |
| |
| 引用本文： | 赵永昕,热依木古丽·阿布都拉,吴 涛,巴 杭,阿吉艾克拜尔·艾萨.高速逆流色谱法从棉花花提取物中分离制备异槲皮素[J].现代药物与临床,2013,28(1):34-36. |
| |
| 作者姓名： | 赵永昕 热依木古丽·阿布都拉 吴 涛 巴 杭 阿吉艾克拜尔·艾萨 |
| |
| 作者单位： | 中国科学院新疆理化技术研究所干旱区植物资源化学重点实验室,新疆乌鲁木齐,830001 |
| |
| 基金项目： | 新疆维吾尔自治区高技术研究与发展项目(201010108) |
| |
| 摘 要： | 目的 应用高速逆流色谱法分离制备异槲皮素.方法 对棉花花提取物用高速逆流色谱法一步分离纯化,以醋酸乙酯-水(1∶1)为两相溶剂体系,上相为固定相,下相为流动相,主机转速为800 r/min,体积流量2.0 mL/min,检测波长254 nm,采用波谱法对所得化合物进行结构鉴定,薄层色谱、高效液相色谱法测定产品的纯度.结果 在该条件下经一步分离可得到质量分数为99％的异槲皮素对照品.结论 该方法操作简便,适合于从棉花花提取物中分离制备异槲皮素对照品.
|
| 关 键 词： | 棉花花提取物 总黄酮 异槲皮素 高速逆流色谱 |
Separation and preparation of isoquercetin from Gossypii Flos extract by high speed counter-current chromatography |
| |
| Institution: | ZHAO Yong-xin,ABDULLA Rahima,WU Tao,BA Hang,AISA Haji Akber Key Laboratory of Plant Resources and Chemistry of Arid,Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Science,Urumqi 830011,China |
| |
| Abstract: | Objective To establish a method for the isolation and preparation of isoquercetin by high speed counter-current chromatography (HSCCC). Methods The total flavonoids in Gossypii Flos extract was further separated by HSCCC with a two-phase solvent system composed of ethylacetate-water (1:1) stationary phase. Main engine speed was 850 r/min and the detection wavelength was 254 nm at a flow rate of 2.0 mL/min. The obtained fraction was identified by spectral analysis and the purity was analyzed by TLC and HPLC. Results Isoquercetin reference substance with the purity of 99% under this given situation was obtained. Conclusion The results indicate that HSCCC is a simple and rapid method for the separation and preparation of isoquercetin reference substance. |
| |
| Keywords: | Gossypii Flos extract total flavonoids isoquercetin high speed counter-current chromatography |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《现代药物与临床》浏览原始摘要信息 |
| 点击此处可从《现代药物与临床》下载免费的PDF全文 |
|
