树突状细胞在肾缺血-再灌注损伤中的分布与作用

目的 :探讨树突状细胞 (DC)在肾缺血再灌注损伤大鼠肾组织中的分布与作用 ,以及抗 P 选择素单克隆抗体 (单抗 )对 DC的影响。方法 :建立肾缺血再灌注损伤大鼠模型 ,随机分为 P选择素单抗治疗组(n=2 0 )和非治疗组 (n=2 0 ) ,按不同再灌注时间 (1、3、6和 2 4 h)再各分为 4组 ;另设假手术组 (n=5 )作为对照。采用荧光图像分析法观察 CD1 a+ CD80 + DC在各组大鼠肾组织中的分布 ;采用免疫组织化学 (组化 )链霉菌抗生物素过氧化物酶复合物 (L SAB)法检测 P 选择素在上述肾组织中的表达。结果 :1 CD1 a+ CD80 + DC在假手术组大鼠肾组织中分布甚少 ;而在非治疗组显著增多 (P<0 .0 0 1 ) ,且主要分布于肾小管、肾间质和肾血管 ,以肾小管间质最为明显 ,其分布和数量自再灌注 1 h起出现增多 ,于 2 4 h时为最高 (P<0 .0 1 ) ,并与大鼠血尿素氮和肌酐呈正相关 (P均 <0 .0 5 )。2缺血再灌注 1 h后 ,P 选择素在肾组织中广泛表达 ,以肾小管上皮细胞为主。 3经抗 P选择素单抗治疗后 ,大鼠肾组织 P选择素的表达下调 ,随之 CD1 a+ CD80 + DC分布及数量减少 (P<0 .0 5和 P<0 .0 1 ) ,继而肾组织病理损伤和肾功能也相应减轻和改善。结论 :DC可能参与了缺血再灌注肾损伤的炎症免疫病理过程 ,并可能与 P选择素介导的肾内黏附

Localization and role of dendritic cells in rat kidney with renal ischemia/reperfusion injury

OBJECTIVE: To investigate the localization and the role of dendritic cells (DCs) in rat kidney with renal ischemia/reperfusion injury, and to explore the effect of anti-P-selectin Lectin-epidermal growth factor (EGF) domain monoclonal antibody (PsL-EGFmAb) on DCs. METHODS:(1)Rat model of renal ischemia/reperfusion was established and rats were divided into treated group with PsL-EGFmAb (n=20) and untreated group (n=20), which included four sub-groups respectively according to reperfusion time (1-hour,3-hour, 6-hour, 24-hour), sham group (n=5) severed as control. CD1a(+)CD80(+)DC was observed with microscopy images method and P-selectin was analysed with immunohistochemistry. RESULTS: (1)In renal tissues of untreated group, CD1a(+)CD80(+)DC was found in renal tubules, interstitium and vessels, especially in renal tubules and interstitium, but DCs were hardly observed in sham group (P<0.001). The number of CD1a(+)CD80(+)DC in 24-hour group was greater than those in other groups (P<0.01). The number of DCs was positively associated with blood urea nitrogen and serum creatinine (both P<0.05). (2)P-selectin content was increased significantly after ischemia/reperfusion 1 hour. (3)The contents of DC and P-selectin were decreased after treated (P<0.05 and P<0.01) in rat renal tissues. CONCLUSION: DCs play an important role in immune pathogenesis after renal ischemia/reperfusion injury, which is related to renal immigration of DCs mediated by P-selectin. PsL-EGFmAb may inhibit local DCs immigration and accumulation in kidney.