高效液相色谱法测定西罗莫司滴眼液的含量

目的建立测定西罗莫司滴眼液含量的高效液相色谱方法。方法采用Diamonsil C18(250 mm×4.6 mm,5μm)为色谱柱;乙腈-甲醇-水(7.5∶62.5∶30,v/v/v)为流动相,柱温50℃,检测波长276 nm,流速1.2 ml/min。结果在20～80μg/ml浓度范围内,西罗莫司峰面积和浓度呈良好的线性关系(r=0.999 7),平均回收率为100.1%,RSD为0.3%,精密度高,耐用性好,样品溶液可以在室温环境下24 h内稳定。结论本方法专属性强,操作简便,结果准确,可用于测定西罗莫司滴眼液的含量。

Determination of Sirolimus Eye Drops by HPLC

Objective To establish an HPLC method for the determination of sirolimus eye drops.Methods The separation was performed on Diamonsil C18(250 mm×4.6 mm,5 μm)column,the mobile phase was acetonitrile-methanol-water(7.5︰62.5︰30) with the flow rate of 1.2 ml/min,the detection wavelength was at 276 nm and column temperature was 50 ℃.Results The calibration curve of sirolimus was linear within the concentration range of 20~80 μg/ml,r=0.999 7.The average recovery rate of sirolimus was 100.1%(RSD=0.3%).Conclusion The method is simple,quick and specific.It can be used to determine the content of sirolimus eye drops.