| 腺苷蛋氨酸对L02细胞尿苷二磷酸葡萄糖醛酸转移酶表达的影响 |
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| 引用本文： | 司徒冰,崔其亮,粱淑文,梅峥嵘. 腺苷蛋氨酸对L02细胞尿苷二磷酸葡萄糖醛酸转移酶表达的影响[J]. 今日药学, 2012, 22(6): 324-325,331 |
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| 作者姓名： | 司徒冰 崔其亮 粱淑文 梅峥嵘 |
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| 作者单位： | 1.广州医学院第三附属医院药学部,广东广州,510150;2.广州医学院第三附属医院儿科,广东广州,510150;3.广州医学院第三附属医院广州医学院,广东广州,510150 |
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| 基金项目： | 2011年广东省医院药学研究基金 |
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| 摘 要： | 目的 探讨腺苷蛋氯酸( S-adenosyl-L-methionine,SAMe)对尿苷二磷酸葡萄糖醛酸转移酶(Uridine Diphosphate Glucuronsyltransferase,UGT)表达的调节作用.方法 体外培养102细胞加入药物分别处理0～72 h后应用RT-PCR法观察SAMe对UGT mRNA的作用.结果 0.5 mmol/L的SAMe在24～ 72 h可上调UGTmRNA的表达,0.1～l mmol/L的SAMe都能上调UGTmRNA的表达,但0.5mmol/L的作用最强,强于苯巴比妥.结论 SAMe可以上调UGTmRNA的表达,从而促进胆红素代谢.
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| 关 键 词： | 腺苷蛋氨酸 尿苷二磷酸葡萄糖醛酸转移酶 表达 |
Effects of S-adenosyl-L-methionine on Expression of Uridine Diphosphate Glucuronsyltransferase in L02 Cells |
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| SITU Bing , CUI Qi-liang , LIANG Shu-wen , MEI Zheng-rong. Effects of S-adenosyl-L-methionine on Expression of Uridine Diphosphate Glucuronsyltransferase in L02 Cells[J]. Pharmacy Today, 2012, 22(6): 324-325,331 |
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| Authors: | SITU Bing CUI Qi-liang LIANG Shu-wen MEI Zheng-rong |
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| Affiliation: | 1(1.Department of Pharmacy,2.Department of Pediatrics,the Third Affiliated Hospital of Guangzhou Medical College,3.Graduate 2009,Guangzhou Medical College,Guangzhou,Guangdong 510150,China) |
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| Abstract: | Objective To detect the function of S-adenosyl-L-methionine(SAMe) on the expression of uridine diphosphate glucuronsyltransferase(UGT).Methods L02 cells were cultured,and they were treated by drugs from 0 to 72 h,respectively.UGTmRNA was detected by RT-PCR.Results SAMe was able to enhance the expression of UGT mRNA from 24 to 72 h.UGTmRNA was regulated by 0.1～1 mmol/L SAMe,and effect of 0.5 mmol/L SAMe was better than phenobarbital.Conclusions SAMe was able to increase the expression of UGTmRNA in L02 cells and promote the metabolism of bilirubin. |
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| Keywords: | S-adenosyl-L-methionine Uridine Diphosphate Glucuronsyltransferase expression |
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