建立去势雌性裸小鼠人子宫内膜癌皮下移植瘤动物模型的探讨

目的:探讨全身外照射对建立切除卵巢后雌性裸小鼠子宫内膜癌皮下移植瘤模型成瘤率的影响.方法:将切除卵巢后1周的裸小鼠随机分为两组,一组全身外照射(4 Gy)3 h后皮下注射子宫内膜癌细胞悬液,另一组未予照射同时间皮下注射子宫内膜癌细胞悬液,观察裸小鼠体质量变化、存活情况和肿瘤的生长情况,测量肿瘤径线并计算体积.结果:2组成瘤率100％;各组肿瘤体积差异无统计学意义(P＞0.05),但存活率差异有统计学意义(P＜0.05).结论:全身外照射不影响切除卵巢的裸小鼠的成瘤率,但增加裸小鼠的死亡率.

The Discussion of Establishing Human Endometrial Carcinoma Xenografts in Ovariectomized Nude Mice

Objective:To discuss the influence of total irradiation on the human endometrial carcinoma xenografts in the ovariectomized nude mice.Methods:One week after the ovariectomy,the female nude mice were randomly distributed into two groups:non-irradiated group and irradiated group.The endometrial cells,which had been cultured in vitro,was subcutaneously transplanted into the nude mice one week after the surgery,in the irradiated group the mice were exposed to 4 Gy of total body irradiation 3 h pior to injection with endometrial cells,in the non-irradiated group the mice were injected with endometrial cells in the same time.the mice weight,survival rate and tumor growth of both groups were observed,the diameters of tumors was measured and the volume of tumors was calculated.Results:The both groups have 100% tumor growth;the difference in tumor sizes is not significant(P<0.05).Conclusions:Total body irradiation did not affect the tumor-take rate on human endometrial carcinoma xenografts in ovariectomized nude mice,but increased the death rate of nude mices.