应用环孢素A治疗异种肝细胞移植的排斥反应

目的　探讨应用环孢素A(CsA)治疗异种肝细胞移植排斥反应的疗效及其排斥机制。方法　将豚鼠肝细胞植入D-氨基半乳糖诱导的肝衰大鼠脾内，分CsA治疗组和单纯移植组。观察2周生存率及脾病理组织学检查；酶联免疫双抗体夹心法(Sandwich-ELISA)测定大鼠血清白细胞介素(IL)-2的浓度。结果　2周生存率比较CsA治疗组（78.6%）与单纯移植组（80.0%）相比差异无显著性(P＞0.05)。应用CsA可减缓炎症细胞浸润。血清IL-2浓度检测正常大鼠为(28.7±7.0)ng/L，移植后各组血清IL-2浓度变化不大，直至移植后第7天,CsA治疗组为(28.5±6.0)ng/L、单纯移植组为(31.5±8.0)ng/L。3组间差异均无显著性(P＞0.05)。结论　在异种肝细胞脾内移植中，细胞免疫发生的较迟和/或较弱。单独应用CsA治疗异种肝细胞脾内移植引发的排斥反应其疗效不佳。

Therapeutic effects of cyclosporine A on the rejection of xenogenic hepatocyte transplantation

Objective　To investigate the immunosuppressive effect of cyclosporine A (CsA) on xenogeneic hepatocyte transplantation (XHT) and the rejection mechanisms. 　Methods　The rats with hepatic failure induced by D-GI were divided into 2 groups: CsA-treated (10 mg/kg per day by intraperitoneal injection) group and CsA-untreated group. The hepatocytes of guinea pig were transplanted into the spleen of the rats. The splenic pathohistological examination in the recipients was performed and the 2-week survival rate in the 2 groups observed. Sandwich-ELISA method was used to determine the serum levels of interleukin-2 in the rats. 　Results　The survival rate in the CsA-treated group （78.6 %） and CsA-untreated group （80.0 %） had no significant difference (P>0.05). But CsA could attenuate the infiltration of inflammatory cells. No significant difference was found in the levels of serum IL-2 of the rats among the normal （28.7±7.0) ng/L, CsA-treated（28.5±6.0) ng/L and CsA-untreated groups ［(31.5±8.0) ng/L, P>0.05］. 　Conclusion　 Cellular immune response would occur later and would be weaker in xenogenic hepatocellular transplantation. CsA has no significant therapeutic effects on XHT.