大鼠急性胰腺炎时ICAM-1在胰、肺组织的表达特征与作用的观察

目的：探讨急性坏死性胰腺炎（ANP）时细胞间粘附分子-1（ICAM-1）在胰腺及肺组织的表达特征和作用。方法。用3.5%的牛磺胆酸钠逆行胰胆管注射制备ANP模型，采用免疫组化技术等方法动态观察ANP时胰腺、肺组织ICAM-1的表达改变和PMN组织聚集的变化。结果：胰腺及肺组织ICAM-1的表达分别在ANP造模后3H、6h明显增强，并于12h达高峰。结论：ICAM-1介导的PMN大量组织聚集可能是ANP早期胰腺及肺组织损伤的重要参与因素。

Polymorphonuclear Neutrophil Infiltration Mediated by Adhesion Molecule in Pancreas and Lung after Acute Necrotizing Panreatitis

Objective:To explore the expressive character and effect of intercellular adhesion molecule-l(ICAM-l)in the pancreas and the lung after acute necrotizing pancreatitis(ANP) Methods:ANP model was induced by retrograde injection of 3.5% sodium taurocholic acid into the biliopancreatic duct.Expression of ICAM-l at different time points in the pancreas and the lung were assessed by immunohistochemistry.The quantity of polymorphonuclear neutrophils(PMN)in tissues was measured by the determination of myeloperoxidase activity(MPO).Results:In ANP,ICAM-l was upregulated evidently at 3 hours in the pancreas and at 6 hours in the lung,reaching their highest levels at 12 hours.Conclusion:The numerous PMNs infiltration mediated by ICAM-l may play an important role of the pancreatic and pulmonary injury in the early phase of ANP.