北京地区乙型肝炎病毒基因型与其感染临床表型的相关性

Association of hepatitis B virus genotypes with clinical phenotypes of hepatitis B virus infection in Beijing

AIM: To investigate the relationship between the geno-types of hepatitis B virus (HBV) and the clinical out-comes of HBV infection in Beijing area.METHODS: A total of 1 321 patients, including those with self limited HBV infection (n = 245), chronic hepatitis B (n = 668), liver cirrhosis (n = 108) and as-ymptomatic carriers (n = 300), were concluded in this study. Serum samples were collected for the detection of HBV markers by enzyme linked immunosorbent as-say (ELISA). A simple and precise genotyping system based on polymerase chain reaction (PCR) using type-specifi c primers was developed for the determination of the genotypes of HBV.RESULTS: Of the 1 321 serum samples, 804 (60.1%)were HBV DNA positive. HBV with the genotype of B, C, and B+C covered a larger percent. The percent-age of HBV with genotype A, B, C, and B+C were 0.25% (2/804), 20.77% (167/804), 69.53% (559/804) and 9.45% (78/804), while none of HBV with geno-type D, E, and F was found. Of all the patients with self limited HBV infection, 14 were HBV DNA positive, and the rates of genotype B, C, and B+C were 28.6% (4/14),64.3% (9/14) and 7.1% (1/14), respectively. Of the samples from asymptomatic carriers, 128 were HBV DNA positive, and the rates of genotype B, C, and B+C were 28.1% (36/128), 68.8% (88/128), and 3.1% (4/128), respectively. Of the patients with in chronic hepatitis B, 608 cases were positive for HBV DNA, and the percentage of genotype B, C, B+C, and A were 19.9% (121/608), 68.6% (417/608), 10.4% (68/608), and 0.3% (2/608), respectively. Of the patients with liver cirrhosis, 54 were HBV DNA positive, and the per-centage of genotype B, C, and B+C were 11.1% (6/54), 88.3% (45/54), and 5.6% (3/54), respectively. The rate of genotype C in liver cirrhosis patients was markedly higher than that in the patients with chronic hepatitis B, self limited HBV infection, and asymptomatic carriers (all P <0.05). In addition, genotype distribution showed no significant differences between the age and sex of patients.CONCLUSION: Genotype B and C are major geno-types in Beijing. HBV with genotype C is predominant in patients with HBV infection, and it may be associ-ated with the development of liver cirrhosis.