| 多重PCR法检测结核分枝杆菌耐药基因研究 |
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| 引用本文： | 谢明章,边文燕,杨清玲,陈昌杰. 多重PCR法检测结核分枝杆菌耐药基因研究[J]. 蚌埠医学院学报, 2011, 36(11): 1173-1176 |
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| 作者姓名： | 谢明章 边文燕 杨清玲 陈昌杰 |
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| 作者单位： | 1. 蚌埠医学院临床检验诊断学实验中心, 安徽蚌埠 233030;2. 蚌埠医学院生化与分子生物学教研室, 安徽蚌埠 233030;3. 山东省淄博市第五人民医院检验科, 山东淄博 256120 |
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| 摘 要： | 目的:建立多重PCR法快速检测结核分枝杆菌耐药基因。方法:采用多重聚合酶链反应(PCR)同时扩增46株结核分枝杆菌临床分离株、48株北京标准菌株、40例耐药及40例非耐药的结核分枝杆菌临床分离菌株的inhA、katG、rpoB、IS1081等基因并采用测序的方法进行验证。结果:所有耐药样本基因(包括利福平、异烟肼耐药基因)及野生型菌株均被成功扩增。结论:应用多重PCR技术可同时快速检测结核分枝杆菌耐药基因。
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| 关 键 词： | 结核分枝杆菌 多重PCR 耐药基因 |
| 收稿时间： | 2010-12-22 |
Multiplex polymerase chain reaction for detection of drug resistance genes of mycobacterium tuberculosis |
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| XIE Ming-zhang,BIAN Wen-yan,YANG Qing-ling,CHEN Chang-jie. Multiplex polymerase chain reaction for detection of drug resistance genes of mycobacterium tuberculosis[J]. Journal of Bengbu Medical College, 2011, 36(11): 1173-1176 |
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| Authors: | XIE Ming-zhang BIAN Wen-yan YANG Qing-ling CHEN Chang-jie |
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| Affiliation: | 1. Clinical Testing and Diagnose Experimental Center, Bengbu Medical College, Bengbu Anhui 233030;2. Department of Biochemistry & Molecular Biology, Bengbu Medical College, Bengbu Anhui 233030;3. Department of Clinical Laboratoty, The Fifth People Hospital of Zibo, Zibo Shandong 256120, China |
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| Abstract: | Objective:To establish a rapid detection method of mycobacterium tuberculosis.Methods:Multiplex polymerase chain reaction(PCR) was employed to dectect katG,IS1081,inhA and rpoB genes of mycobacterium tuberculosis.Forty-eight DNA samples were obtained from the national institute for the control of pharmaceutical and biological products(NICPBP),forty drug resistant mycobacterium tuberculosis were isolated and 40 drug sensitive mycobacterium tuberculosis were isolated from the culture-based bacterias and 46 fr... |
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| Keywords: | mycobacterium tuberculosis multiplex polymerase chain reaction drug resistance genes |
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