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| 染色法鉴别旋毛形线虫成虫死活的实验观察 | |
| 引用本文： | 王小莉,李亮,方强,崔洁,郭凯,沈继龙.染色法鉴别旋毛形线虫成虫死活的实验观察[J].蚌埠医学院学报,2011,36(10):1041-1042,1046. |
| 作者姓名： | 王小莉 李亮 方强 崔洁 郭凯 沈继龙 |
| 作者单位： | 1. 安徽医科大学,病原生物学教研室,安徽,合肥,230032;蚌埠医学院,病原生物学教研室,安徽省感染与免疫重点实验室,安徽,蚌埠,233030 2. 蚌埠学院,食品与生物工程系,安徽,蚌埠,233000 3. 蚌埠医学院,病原生物学教研室,安徽省感染与免疫重点实验室,安徽,蚌埠,233030 4. 安徽医科大学,病原生物学教研室,安徽,合肥,230032 |
| 基金项目： | 国家重点基础研究发展计划(973计划)资助项目(2010CB530000) |
| 摘 要： | 目的:探讨快速鉴别旋毛形线虫成虫死活的染色方法.方法:分别以0.5%番红、1.0%中性红和0.5%甲基蓝3种染液,对死或活旋毛形线虫成虫进行染色.结果:0.5%番红、1.0%中性红染液能使死亡旋毛形线虫成虫着色;旋毛形线虫活成虫对3种染液均不着色.结论:0.5%番红、1.0%中性红染液染色均可快速鉴别旋毛形线虫成虫死活... |
| 关 键 词： | 旋毛形线虫 成虫 番红 中性红 甲基蓝 |
Experimental study on determination of viability of the adult of Trichinella spiralis in vitro |
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| WANG Xiao-li,LI Liang,FANG Qiang,CUI Jie,GUO Kai,SHEN Ji-long.Experimental study on determination of viability of the adult of Trichinella spiralis in vitro[J].Journal of Bengbu Medical College,2011,36(10):1041-1042,1046. | |
| Authors: | WANG Xiao-li LI Liang FANG Qiang CUI Jie GUO Kai SHEN Ji-long |
| Institution: | WANG Xiao-li1,2,LI Liang3,FANG Qiang2,CUI Jie2,GUO Kai2,SHEN Ji-long1(1.Department of Microbiology and Parasitology,Anhui Medical University,Hefei Anhui 230032,2.Department of Microbiology and Parasitology,Anhui Provinical Key Laboratory of Infection and Immunity,Bengbu Medical College,Bengbu Anhui 233030,3.Department of Food and Biological Engineering,Bengbu College,Bengbu Anhui 233000,China) |
| Abstract: | Objective:To explore the prompt methods in determination of viability of the adult of Trichinella spiralis.Methods:The adult worms of Trichinella spiralis were stained with 0.5% safranine,1.0% neutral red or 0.5% methylene blue.Results:The dead adults were observed when stained with 0.5% safranine or 1.0% neutral red in the study.The vital adults were unstained with all three stains.Conclusions:Both 0.5% safranine staining and 1.0% neutral red staining are prompt method in determination of viability of the ... |
| Keywords: | Trichinella spiralis imago safranine neutral red methylene blue |
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