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血涂片染色方法的改良
引用本文:李璐. 血涂片染色方法的改良[J]. 华夏医学, 2011, 24(3): 351-352
作者姓名:李璐
作者单位:广东医学院组织胚胎学教研室,广东湛江,524023
摘    要:目的:探索提高血涂片细胞形态和颜色以及持久性的制作方法。方法:实验从染色冲洗的环节进行改良,采用蒸馏水(纯净水)取代自来水。结果:血涂片用Wright-Giemsa染色、蒸馏水(纯净水)冲洗的方法在光镜下观察细胞结构较清晰,层次分明,不易褪色,保存和使用时间较长。结论:该方法提高了血涂片染色的质量。

关 键 词:血涂片  Wright-Giemsa混合染色  蒸馏水

The modification of blood smear staining methods
LI Lu. The modification of blood smear staining methods[J]. Acta Medicinae Sinica, 2011, 24(3): 351-352
Authors:LI Lu
Affiliation:LI Lu Guangdong Medical College,Zhanjian 524023,China
Abstract:Objective:To explore the blood smear staining method,which allows good cell shape and color.Methods:The staining method and washing step were reformed and tap-water was replaced by double distilled water.Results:Cell structure and distinct gradations were clearly observed under microscopy with Wright-Giemsa mixed staining and double distilled water washing.Conclusion:This method is not easy to fade and suitable for long-term use and preservation,improving the quality of blood smeas staining.
Keywords:blood smear  Wright-Giemsa staining  distilled water  
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