黑木耳粉对矽肺大鼠肺内胶原合成的影响及其抗氧化应激机制

目的：探讨黑木耳粉对染矽尘大鼠肺组织胶原合成的抑制作用及其对抗氧化物酶活性和脂质过氧化水平的影响。方法：将80只Wister大鼠随机分为阴性对照组、染矽尘组、黑木耳粉干预高、中、低剂量组共5个组,每组16只,采用非暴露式气管插管方法给大鼠染二氧化硅（SiO2）粉尘,以喂饲途径给予黑木耳粉,分别于第14、56 d时,采用HE染色对矽肺纤维化病变进行形态学观察,采用羟脯氨酸（HYP）测定法对矽肺大鼠肺内胶原含量进行检测,分别采用硫代巴比妥酸法、黄嘌呤氧化酶法测肺内丙二醛（MDA）含量及超氧化物歧化酶（SOD）活性。结果：14 d时,黑木耳粉各剂量组与染矽尘组相比,病理形态改变有不同程度的减轻,病理分级减轻1/2级,HYP含量均低于染尘组,高、中剂量组与染尘组比较差异有统计学意义（P〈0.05）,SOD活性各剂量组均高于染尘组（P〈0.05）,MDA含量各剂量组均低于染尘组,高、中剂量组与染尘组比较差异有统计学意义（P〈0.05）;56 d时,黑木耳粉各剂量组比染矽尘组病理形态改变均有所减轻,病理分级减轻1/2级,黑木耳粉高剂量组HYP含量仍明显低于染尘组（P〈0.05）,高剂量组SOD活性仍明显高于染尘组（P〈0.05）,且MDA含量仍明显低于染尘组（P〈0.05）。结论：黑木耳粉能够明显降低矽肺大鼠肺内胶原蛋白的含量,对矽肺组织纤维化有一定的阻抑作用,其机制可能是通过提高抗氧化物酶SOD的活力、减轻脂质过氧化损伤,提高机体的抗氧化损伤能力而发挥抗肺纤维化作用。

Effects of Auricularla Auricula Powder on Collagen Synthesis in Silicotic Rats and its Mechanism of Antioxidative Stress

Objective: To explore the effects of Auricularia auricular powder(AAP) on the collagen synthesis,antioxidase activities,and levels of lipid peroxidation in silicotic rats.Methods: Eighty rats were randomly divided into control group,silica group,and AAP-treated groups with high,medium and low doses.Each group had 16 rats.In AAP-treated groups,the animal models were established by direct tracheal instillation of silica,and after 24 hours,AAP were given by feeding.At the 14th and 56th day,Lung fibrosis in mor...