过量维A酸诱导昆明小鼠露脑畸形动物模型的建立及其形态学观察

目的: 建立过量维A酸(RA)诱导的露脑畸形动物模型,分析RA在胚胎发育期间对胎鼠和母孕鼠的毒性作用。方法: 将昆明小鼠随机分为对照组20只和实验组60只,实验组分别在妊娠(GD)7.50 d、GD7.75 d、GD8.00 d和GD8.50 d一次性灌服相应剂量(20、30、40和50 mg/kg)的全反式RA (atRA)花生油悬液,对照组灌服等剂量花生油。结果: 不同用药时间和不同剂量的atRA均可致露脑畸形发生,且其发生率随用药剂量的增加而增加、随孕龄的增加而减少。其中GD 7.75 d 30 mg/kg实验组诱导露脑畸形发生率最高。GD 7.75 d 30 mg/kg RA组显示头部神经管未闭,GD 16.50 d胚胎可见头部缺损处脑组织外露,颅顶骨及头部皮肤发育不全。结论: 成功建立atRA致昆明小鼠露脑畸形动物模型,为进一步研究露脑畸形的发生机制奠定了基础。

The establishment of exencephaly model induced by retinoic acid and its morphological observation in KM mice

Objective:To set up the retinoic acid(RA)-induced exencephaly model in KM mice,and analyze the toxicity of RA to fetus and its mother.Methods:The KM mice were randomly divided into test and control groups.The mice were orally treated with a single dose of 20,30,40 and 50 mg/kg of all-trans-RA which dissolved in peanut oil at a selective time of gestational day(GD)7.50 d,GD7.75 d,GD8.00 d or GD8.50 d.The mice in control group were administrated with a volume of peanut oil,equal to the dose of RA.Results:RA c...