Monoclonal and xenoantibodies specific for HLA-DR inhibit primary responses to HLA-D but fail to inhibit secondary proliferative (PLT) responses to allogeneic cells |
| |
| Authors: | D D Eckels J N Woody R J Hartzman |
| |
| Affiliation: | 1. Division of Immunologic Oncology, Department of Pediatrics, Lombardi Cancer Center, Georgetown University School of Medicine, Washington, D.C., USA;2. Tissue Bank, Naval Medical Research Institute, Bethesda, Maryland, USA |
| |
| Abstract: | ![]()
Monoclonal antibodies (DA-2 and CA-2) and xenoantisera (rabbit anti-human, p23,30) specific for HLA-DR framework determinants were added to primary and secondary mixed lymphocyte cultures. Although such antisera were shown to inhibit primary MLC, primed lymphocytes were much less sensitive to the blocking effects of these antibodies. In the studies shown here, the concentration of antibody required to inhibit primary MLC reactions was 0.1-1.5 micrograms/ml). |
| |
| Keywords: | HLA human major histocompatibility complex MLR mixed lymphocyte reaction MLC mixed lymphocyte culture PLT primed lymphocyte typing PBL peripheral blood lymphocytes HTC homozygous typing cells DNV double normalized values |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
