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Trehalose attenuates spinal cord injury through the regulation of oxidative stress,inflammation and GFAP expression in rats |
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| Authors: | Mahdieh Nazari-Robati Mahboobe Akbari Mohammad Khaksari Moghaddameh Mirzaee |
| Affiliation: | 1. Department of Clinical Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran;2. Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran;3. Endocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran;4. Department of Biostatistics and Epidemiology, School of Public Health, Kerman University of Medical Sciences, Kerman, Iran |
| Abstract: | Objective: Inflammation and oxidative stress are implicated in pathogenesis of spinal cord injury (SCI). Trehalose, a nonreducing disaccharide, exhibits anti-inflammatory and antioxidant effects. The present study investigated the therapeutic efficacy of trehalose in the SCI model. Design and setting: An experimental study was designed using 120 male Wistar rats which were randomly divided into three groups including SCI, SCI?+?phosphate buffer saline (vehicle) and SCI?+?trehalose. All rats were subjected to SCI. Immediately after SCI, vehicle and trehalose groups received intrathecal injection of buffer and trehalose, respectively. Outcome measures: The level of tissue TNFα, IL-1β, nitric oxide, malondialdehyde, myeloperoxidase, glial fibrillary acidic protein (GFAP) as well as hindlimb function were assessed at 4 hours, 1, 3 and 7 days post-SCI. Results: Data indicated an early significant decrease in inflammatory and oxidative responses following SCI in trehalose treated group. Moreover, trehalose reduced GFAP expression as soon as 1-day post-trauma. Furthermore, trehalose treatment increased the score of hindlimb function. Conclusion: Our results indicated that treatment with trehalose reduces the development of secondary injury associated with SCI. This effect likely underlies improved neurological function. |
| Keywords: | Spinal cord injury Trehalose Inflammation Oxidative stress Reactive astrocytes Locomotor function |