| 缺氧-再给氧时PDTC对血管内皮细胞选择素E表达的作用 |
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| 引用本文： | 郑立新,安琪,石应康. 缺氧-再给氧时PDTC对血管内皮细胞选择素E表达的作用[J]. 中华胸心血管外科杂志, 2004, 20(3): 161-163 |
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| 作者姓名： | 郑立新 安琪 石应康 |
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| 作者单位： | 610041,成都,四川大学华西医院胸心血管外科 |
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| 基金项目： | 国家自然科学基金资助 (39870 748) |
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| 摘 要： | 目的探讨抗氧化剂PDTC对血管内皮细胞(EC)选择素E(E-selectin)转录的抑制作用。方法以人脐静脉血管内皮细胞为材料，建立缺氧-再给氧模型，检测E-selectin的表达；应用PDTC干预EC的缺氧培养过程，观察E-selectin表达的改变。结果EC缺氧-再给氧反应后E-selectin的表达明显增加，4h达到峰值；PDTC能有效抑制E-selectin的表达。结论缺氧-再给氧促进EC活化，增强E-selectin的表达，抗氧化剂PDTC能有效降低E-selectin的转录，为心肌缺血-再灌注损伤的治疗提供理论基础。
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| 关 键 词： | 缺氧 给氧 PDTC 血管内皮细胞 选择素E 抗氧化剂 吡咯烷二硫氨基甲酸酯 心肌缺血 再灌注损伤 心肌保护 |
PDTC reduces E-selectin expression on hypoxia/reoxygenation-stimulated endothelial cells |
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| ZHENG Li xin,AN Qi,SHI Ying kang. PDTC reduces E-selectin expression on hypoxia/reoxygenation-stimulated endothelial cells[J]. Chinese Journal of Thoracic and Cardiovascular Surgery, 2004, 20(3): 161-163 |
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| Authors: | ZHENG Li xin AN Qi SHI Ying kang |
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| Affiliation: | ZHENG Li xin,AN Qi,SHI Ying kang. Department of Cardio Thoracic Surgery,West China Hospital,Sichuan University,Chengdu 610041,China |
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| Abstract: | Objective: To study the mechanisms and treatment of ischemia/reperfusion injury. Expression of E selectin was measured and the effect on suppression of E selectin by the Pyrrolidine Dithiocarbamate was investigated. Methods: Endothelial cells were exposed to hypoxia, then returned to reoxygenation condition. ELISA methods were used to detect expression of E selectin. Results: E selectin expression on hypoxia/reoxygenation stimulated endothelial cells increased, PDTC could suppress translation of E selectin effectively. Conclusion: It seems that E selectin may act as a critical factor. PDTC may prove beneficial in the treatment of ischemia/reperfusion injury. |
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