Evaluation of a reverse line blot assay for genotyping common human rotaviruses |
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| Authors: | Erwin Duizer Bas van der Veer Erwin de Bruin Mark Zeller Elisabeth Heylen Marc van Ranst Jelle Matthijnssens Marion Koopmans Harry Vennema |
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| Affiliation: | 1. Division for Virology, Centre for Infectious Diseases Control, National Institute for Public Health and the Environment (RIVM), P.O. Box 1, 3720 BA Bilthoven, The Netherlands;2. Laboratory of Clinical and Epidemiological Virology, Department of Microbiology and Immunology, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium;3. Virology Department, Erasmus Medical Center, Rotterdam, The Netherlands |
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| Abstract: | ![]()
To allow high-throughput genotyping of group A rotaviruses (RVA) in a routine surveillance setting, we developed a reverse line blotting method for the determination of the most common human RVA G- and P-genotypes: G1–G4, G9, G12, P[4], P[6] and P[8]. Using the reverse line blotting method on 951 clinical RVA positive feces samples, in 905 (95%) of the samples the G-genotyping yielded a result while in 945 (99%) of the samples the P-genotyping was successful. Comparison of the reverse line blotting-method as it is used currently to a sequence based method for genotyping RVAs showed an agreement of 96% for single strain infections (75 out of 78) but only 48% for mixed infections (10 out of 21). |
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| Keywords: | Rotavirus Genotyping Reverse line blot |
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