| Folin-Ciocalteu比色法测定枸杞子中总酚酸的含量 |
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| 引用本文： | 王晓宇,陈鸿平,银玲,刘友平. Folin-Ciocalteu比色法测定枸杞子中总酚酸的含量[J]. 亚太传统医药, 2012, 8(1): 45-47 |
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| 作者姓名： | 王晓宇 陈鸿平 银玲 刘友平 |
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| 作者单位： | 1. 成都中医药大学药学院,四川成都611137;中药材标准化教育部重点实验室,四川成都611137
2. 成都中医药大学药学院,四川成都611137;中药材标准化教育部重点实验室,四川成都611137;中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137 |
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| 基金项目： | 国家中医药管理局中医药行业科研专项 |
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| 摘 要： | 目的:采用Folin-Ciocalteu比色法测定枸杞子中总酚酸的含量。方法:以绿原酸作为对照品,以Folin-Ciocalteu试剂为显色剂,检测波长为761nm。结果:浓度为0.00318～0.0212mg/mL时与吸光度呈良好线性关系,测定方法的平均回收率为100.8%,相对标准偏差(RSD)为0.71%。结论:此法操作简便,准确可靠,为枸杞子药材的质量控制、综合开发利用提供了一定的方法和依据。
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| 关 键 词： | 枸杞子 Folin-Ciocalteu比色法 总酚酸 |
Determination of Total Phenolic Acid in the Fruits of Lycium barabarum L.by Folin-Ciocalteu Colorimetric Method |
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| Wang Xiaoyu,Chen Hongping,Ying Ling,Liu Youping. Determination of Total Phenolic Acid in the Fruits of Lycium barabarum L.by Folin-Ciocalteu Colorimetric Method[J]. Asia-Pacific Traditional Medicine, 2012, 8(1): 45-47 |
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| Authors: | Wang Xiaoyu Chen Hongping Ying Ling Liu Youping |
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| Affiliation: | 1,2,3(1.College of Pharmacy,Chengdu University of Traditional Chinese Medicine; 2.The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine; 3.State Key Laboratory Breeding Base of Systematic Research,Development and Utilization of Chinese Medicine Resources,Chengdu 611137,China) |
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| Abstract: | Objective:The content of total Phenolic acid in the Fruits of Lycium barabarum L.was determined by Folin-Ciocalteu Colorimetric Method.Methods:With chlorogenic acid as the reference substance,Folin-Ciocalteu reagent was used as the chromogenic agent,The detection wavelength was 761nm.Results:It showed a good linear relationship between the concentration and the absorbance in the range of 0.00318~0.0212mg/ml,the average recovery rate of the added sample was 100.8%,the relative standard deviation(RSD) of 0.71%.Conclusion:The Method is simple,convenient,accurate and reliable.It can be provided certain method and basis for the quality control,comprehensive development and utilization of the Fruits of Lycium barabarum L. |
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| Keywords: | the Fruit of Lycium barabarum L. Folin-Ciocalteu Colorimetric Method Total Phenolic Acid |
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