胸苷酸合成酶的表达及其与胃癌的相关性研究

目的探讨胃癌组织中胸苷酸合成酶(TS)的表达对胃癌患者预后的影响。方法采用免疫组织化学染色的方法(ABC法)检测了164例胃癌(ⅠB～Ⅳ期)石蜡标本中的TS基因的表达情况。结果164例标本中TS基因表达的阳性率为48.8%(80/164),TS的表达与肿瘤分化程度、浸润深度、淋巴结转移与否及临床病理分期密切相关(P<0.001),且TS表达阴性患者的5年生存率(52.4%)明显高于TS表达阳性者(12.3%,P<0.001)。多因素分析显示,肿瘤的病理分化程度、浸润深度和TS表达状态3者与生存率有相关性(P<0.05),其中TS的表达状态与患者5年生存率关系最为密切(P<0.0001),相对危险系数为1.197。结论胃癌组织中TS表达水平的高低可能是胃癌患者一个重要的预后指标,对临床医生选择化疗方案具有一定的指导意义。

Expression of thymidylate synthase and its relationship with gastric carcinoma

Objective To study the expression of thymidylate synthase(TS) gene in gastric carcinoma and its relationship with the prognosis. Methods Expression of thymidylate synthase was detected by immunohistochemistry in 164 gastric carcinoma tissues. Its relationship with clinicopathological parameters and prognosis was analyzed. Results The positive rate of TS expression was 48 8%(80/164). TS expression was significantly correlated with four clinicopathologic parameters: histologic type, depth of invasion, lymph node metastasis and clinical stage(P< 0 001). The 5 year survival rate was significantly higher in TS negative group(52 4%) than that in TS positive group(12.3%)(P< 0.001).A multivariate analysis revealed that histologic type, depth of invasion and TS expression were independent prognostic factors (P< 0.05),especially TS expression (P< 0.0001). The relative hazard ratio of TS grade was 1.197. Conclusions The expression of TS may be one of the important prognostic indicator for gastric carcinoma and a relatively reliable indicator for whether 5 FU should be used in the treatment of gastric carcinoma.