不同产地斑蝥超微粉体的HPLC指纹图谱及斑蝥素的含量测定

目的:结合斑蝥主要成分斑蝥素的含量测定与指纹图谱,比较不同产地药材化学成分的差异性,为科学评价不同产地斑蝥药材及其超微粉体的质量提供依据。方法:建立斑蝥超微粉体指纹图谱检测方法。采用高效液相色谱(HPLC)法对各产地斑蝥超微粉体进行分析,采用中药色谱指纹图谱相似度评价系统软件比较指纹图谱的相似度,采用SPSS 13.0统计软件对10批样品进行聚类分析,并测定斑蝥素含量。结果:不同产地斑蝥超微粉体指纹图谱有较大相似性,但斑蝥素的含量有一定的差异。聚类分析结果表明,不同产地斑蝥药材存在一定差异,但差别不大。结论:所建立的HPLC指纹图谱具有较好的精密度、重复性和稳定性,结合斑蝥素的含量,可为斑蝥药材及其超微粉体质量评价提供依据。

Study on the HPLC Fingerprints of Spanish fly Ultra-fine Powder from Different Producing Areas and Content Determination of Cantharidin

OBJECTIVE：To compare the difference of chemical components in Spanish fly from different producing areas on the basis of content determination and fingerprints of cantharidin,and to provide reference for quality control of S. fly from different producing areas and its ultra-fine powder. METHODS：Established the fingerprint method of S. fly ultra-fine powder. S. fly ultra-fine powder were determined by HPLC. Chromatographic Fingerprint Similarity Evaluation software was used to analyze the similarity of HPLC fingerprint. Cluster analysis was conducted in 10 batches of sample by using SPSS 13.0 software,and the content of cantharidin was determined. RESULTS：The fingerprints of S. fly ultra-fine powder from different producing areas were similar to each other. The content of cantharidin had little difference. Cluster analysis showed that there was little difference in S. fly from different producing areas. CONCLUSIONS：Established HPLC fingerprint is precise,reproducible and stable,and combined with content determination of cantharidin to provide reference for quality evaluation of S. fly and its ultra-fine powder.