从“木郁克土”角度探讨围绝经期综合征肝郁证大鼠胃肠动力的改变

目的从“木郁克土”角度探讨围绝经期综合征(PPS)肝郁证大鼠胃肠动力的改变。方法选择3月龄SPF级SD雌性大鼠,分为假手术组、PPS组、PPS肝郁组和柴胡疏肝散组,采用去势法结合慢性不可预知性温和应激建立PPS肝郁证大鼠模型,用柴疏疏肝散进行干预,观察大鼠体重增长率、饲料消耗量、胃残留率及肠推进率变化。结果在体重增长率方面,PPS组显著高于假手术组(P<0.01);PPS肝郁组显著低PPS组(P<0.01);柴胡疏肝散组显著高于PPS肝郁组(P<0.01)。在饲料消耗方面,PPS组高于假手术组,PPS肝郁组低于PPS组;柴胡疏肝散组高于PPS肝郁组。在胃残留率方面,PPS肝郁组显著高于假手术组和PPS组(P<0.01);柴胡疏肝散组显著低于PPS肝郁组(P<0.01)。在肠推进率方面,PPS肝郁组显著低于假手术组和PPS组(P<0.01);柴胡疏肝散组显著高于PPS肝郁组(P<0.01)。结论PPS肝郁大鼠的体重和饲料消耗量发生了改变,体重的增长与饲料的消耗量存在一定的联系,且与大鼠胃肠动力有关。从“木郁克土”角度提示其机制可能是肝郁状态影响到了脾胃功能的正常运行。

Changes of gastrointestinal motility in perimenopause syndrome rats with liver depression from the point of "mu-yu-ke-tu"

Objective To explore the influence of perimenopause syndrome(PPS)with liver depression on spleen and stomach by observing the change of gastrointestinal motility.Methods 3-month-old SPF SD female rats were selected and divided into sham,PPS,PPS with liver depression and Chaihu shugansan powder groups.The rat model of PPS with liver depression was established by castration combined with chronic unpredictable mild stress.Chaihu shugan powder was used for intervention.The changes of weight growth,food consumption,gastric residual rate and intestinal propulsion rate of rats were observed.Results In terms of weight growth rate,PPS group was significantly higher than that of sham group(P<0.01),PPS with liver depression group was lower than that of PPS group(P<0.01),and Chaihu shugan powder group was higher than that of PPS with liver depression group(P<0.05).In terms of food consumption,PPS group was significantly higher than that of sham group,PPS with liver depression group was significantly lower than that of PPS group,Chaihu shugan powder group was higher than that of PPS with liver depression group.In terms of gastric residual rate,PPS with liver depression group was significantly higher than that of sham group(P<0.05),and there was no statistical difference between Chaihu shugan powder group and PPS with liver depression group.In terms of intestinal propulsion rate,PPS with liver depression group was significantly lower than that of sham group(P<0.01),and was significantly lower than that of Chaihu shugan powder group(P<0.01).Conclusions Weight and food consumption have changed in PPS with liver depression rats.The increase of weight is related to the food consumption and gastrointestinal motility.From the point of"mu-yu-ke-tu",the possible mechanism might be liver depression affecting the function of spleen and stomach.