| HPLC法测定杠板归中槲皮素和山柰素的含量 |
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| 引用本文： | 陈建,朱彩芳,蔡芳,殷黎霞,莫晶昉,郦红岩. HPLC法测定杠板归中槲皮素和山柰素的含量[J]. 现代中药研究与实践, 2013, 0(6): 69-70 |
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| 作者姓名： | 陈建 朱彩芳 蔡芳 殷黎霞 莫晶昉 郦红岩 |
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| 作者单位： | 江苏吉贝尔药业有限公司,江苏镇江212009 |
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| 摘 要： | 目的建立杠板归中槲皮素和山柰素含量的HPLC测定方法。方法色谱柱:Kromasil C18(5μm,250×4.6mm);流动相:甲醇-0.4%磷酸溶液(60:40);检测波长:360nm,柱温:30℃。结果槲皮素和山柰素的回收率分别为99.6%、100.8%;线性范围分别为0.158 93.178μg、0.006 993.178μg、0.006 990.279 6μg。结论结果准确,重复性好,可用于该产品的质量控制。
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| 关 键 词： | 杠板归 槲皮素 山柰素 含量测定 HPLC |
Determination of Quercetin and Kaempferol in Polygonum perfoliatum L. by HPLC |
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| CHEN Jian,ZHU Cai-fang,CAI Fang,YIN Li-xia,MO Jin-fang,LI Hong-yan. Determination of Quercetin and Kaempferol in Polygonum perfoliatum L. by HPLC[J]. Research and Practice on Chinese Medicines, 2013, 0(6): 69-70 |
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| Authors: | CHEN Jian ZHU Cai-fang CAI Fang YIN Li-xia MO Jin-fang LI Hong-yan |
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| Affiliation: | (Jiangsu Jibrier Pharm Co., Ltd.,Zhenjiang 212009,China) |
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| Abstract: | Objective To establish an HPLC method for the determination of quercetin and kaempferol in Polygonum perfoliatum L.Methods HPLC was used to quantitative analysis. The Kromasil C18 column（5pm,250×4.6mm） was used,and mobile phase was composed of methanol-0.4%H3PO4/Water （ 60：40 ） . Detection wavelength was at 360 nm at 30℃. Results The quercetin and kaempferol＇s average recovery were 99.6% and 100.8%,respectively. Linear range of methods was 0.158 9-3.178μg and 0.006 99-0.279 6μg, respectively.Conclusion The result is accurate and the control of Polygonum perfoliatum L. reproducibility is good. The method can be used for quality |
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| Keywords: | Polygonumperfoliatum L. quercetin kaempferol determination HPLC |
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