普瑞巴林对大鼠慢性炎性疼痛治疗过程中吗啡急性耐受的抑制作用

目的:观察吗啡在治疗大鼠慢性炎性疼痛的过程中是否会发生急性耐受,以及普瑞巴林对该急性耐受是否具有抑制作用。方法:成年健康雄性SD大鼠,随机分为3组(n=8)。以完全福氏佐剂注射到大鼠左侧后肢足底皮下,制成慢性炎痛模型。造模成功后第3天开始,连续3 d灌胃分别给予吗啡8 mg/kg(M组)、普瑞巴林3 mg/kg(P组)、吗啡8 mg/kg和普瑞巴林3 mg/kg(MP组)。采用von Frey仪测定大鼠患足机械刺激缩足阈值,采用热板测痛仪测定大鼠的热刺激缩足潜伏期。比较每组大鼠每天给药前及给药后第15、30、60及120 min的机械刺激缩足阈值及热刺激缩足潜伏期变化。结果:M组大鼠第1天给药后各时点及第2天给药后第15 min的机械刺激缩足阈值及热刺激缩足潜伏期均显著高于当日给药前,差异有统计学意义(P<0.01或P<0.05);第2天给药后第30、60、120 min及第3天给药后各时点的机械刺激缩足阈值及热刺激缩足潜伏期与当日给药前相比无统计学意义。P组大鼠连续3 d给药前后的机械刺激缩足阈值及热刺激缩足潜伏期均无统计学意义。MP组大鼠第1天给药后各时点及第2天给药后第30、60及120 min的机械刺...

Depression of pregabalin against acute tolerance of morphine in rats with chronic inflammatory pain

AIM: To investigate if pregabalin could depress the acute tolerance induced by morphine when curing the chronic inflammatory pain of rats.METHODS: 24 adult male Sprague-Dawley rats weighing 180-200 g were randomly divided into three groups(8 in each group).Freund s adjuvant was injected subcutaneously in left hind-paw of the rat to establish the chronic inflammatory pain model.3 days later,rats received 3 daily intragastrical(p.o.) morphine 8 mg/kg(M group),pregabalin 3 mg/kg(P group) or morphine 8 mg/kg+pr...