安多霖对微波辐射致大鼠脑损伤的预防作用研究

目的 探讨安多霖对微波辐射致大鼠脑损伤的预防作用.方法 140只二级雄性Wiser大鼠随机分为5组:健康对照组、辐射对照组、低浓度(0.75 g·kg～·d-1)预防组、中浓度(1.5 g·kg～·d-1)预防组及高浓度(3 g·kg～·d-1)预防组.预防组每日1次灌胃给予安多霖溶液,连续给药工2周.给药结束后采用30 mW/cm.微波辐射大鼠15 min.于处理后6 h、7 d和14 d,采用Morris水迷宫检测大鼠学习和记忆能力,高效液相色谱检测海马氨基酸类神经递质含量,光镜和电镜观察海马组织学和超微结构变化.结果 微波辐射后7 d内,大鼠学习和记忆能力下降(F=0.000～0.043,P<0.05);微波辐射后6 h,4种氨基酸类神经递质含量均降低,其中谷氨酸、甘氨酸及γ-氨基丁酸降低明显(F=0.000～0.007,P<0.01);微波辐射后6 h、7 d,海马组织水肿,神经元变性;神经元线粒体肿胀、空化,内质网扩张,突触间隙模糊,血管周间隙增宽.低浓度预防组上述变化与辐射对照组相似.中浓度和高浓度预防组微波辐射后7 d内,大鼠学习和记忆能力损伤不明显,两者与辐射对照组相比,差异有统计学意义(F=0.015～0.028,P<0.05);微波辐射后6 h,4种氮基酸类神经递质含量均无明显下降,其中谷氨酸含量接近正常,两者与辐射对照组比,差异有统计学意义(F=0.000-0.042,P<0.05);微波辐射后6 h、7 d,海马组织无明显损伤.结论 30 mW/cm.微波辐射可引起大鼠学习和记忆能力下降、海马氨基酸类神经递质代谢紊乱及海马组织学和超微结构损伤;1.5和3 g·kg-1·d-1安多霖对微波辐射致大鼠脑损伤有预防作用.1.5 g·kg-1·d-1安多霖为预防微波辐射致大鼠脑损伤的有效剂量.

Abstract：

Objective To study the prevention effects of AduoLa Fuzhenglin(ADL)Oll the brain injury induced by microwave radiation in rats.Methods A total of 140 male Wismr rats were divided randomly into 5 groups,including control group,microwave exposed group,low dosage(0.75 g·kg-1·d-1)group.middle dosage(1.5 g·kg-1·d-1)group and high dosage(3 g·kg-1·d-1)group.Rats in three ADL groups were lavaged with ADL per day for 2 weeks before radiation.After administration,rats were exposed to microwave at 30 mW/cm2 for 15 min.The abilities of learning and memory were detected by Morris water maze,and the contents of amino acids neurotransmitter of hippocampus were detected by HPLC, then the histology and uhrastrncture of hippocampus were observed with light and electron microscope at 6 h,7 and 14 d after exposure.Results The abilities of learning and memory were declined(F=0.000-0.043,P<0.05)from 6 h to 7 d after exposure,and the contents of four kinds of amino acid neurotransmitter in hippocampus were decreased,of which GLU,GLY and GABA were decreased significantly(F=0.000-0.007,P<0.01)at 6h after exposure,then tissue edema,neuronal degeneration,neuron mitoehondria swelling and cavitation,endocytoplasmie rotieulum broaden,synaptic cleft blurred,and perivascular space widen were found in the hippocampus at 6 h and 7 d after exposure.The changes in low dosage group were similar to those of the radiation group.However,in middle and high dosage groups,the abilities of learning and memory were normal to some extent with the significant differences compared to the radiation group from 6 h to 7 d after exposure(F=0.015-0.028.P<0.05).The contents of four kinds of amino acid neurotransmitter were not decreased,especially GLU contents close tO normal level.There were significant differences between middle and high dosage groups and radiation group at 6 h after exposure(F=0.000-0.042,P<0.05).Moreover,no obvious injury in the hippocampus was observed in middle and high dosage groups at 6 h and 7 d after exposure.Conclusions Exposure to 30 mW/cm2 microwave radiation could decrease the abilities of learning and memory,induce amino acid neurotransmitter turbulence,and injure the histology and uhrastructure of hippocampus.ADL at the dosages of 1.5 and 3 g·kg-1·d-1 would have preventive effects on the injury induced by microwave exposure.The concentration of 1.5 g·kg-1 ·d-1 of ADL might be the effective dosage to prevent the brain damage after microwave exposure.

Prevention effects of AduoLa Fuzhenglin on brain injury induced by microwave radiation in rats

Objective To study the prevention effects of AduoLa Fuzhenglin (ADL) on the brain injury induced by microwave radiation in rats. Methods A total of 140 male Wistar rats were divided randomly into 5 groups, including control group, microwave exposed group, low dosage (0.75 g ·kg^-1 ·d^-1 ) group, middle dosage (1.5 g ·kg^-1 ·d^-1) group and high dosage(3 g ·kg^-1 ·d^-1) group. Rats in three ADL groups were lavaged with ADL per day for 2 weeks before radiation. After administration, rats were exposed to microwave at 30 mW/cm² for 15 min. The abilities of learning and memory were detected by Morris water maze, and the contents of amino acids neurotransmitter of hippocampus were detected by HPLC, then the histology and ultrastructure of hippocampus were observed with light and electron microscope at 6 h, 7 and 14 d after exposure. Results The abilities of learning and memory were declined (F=0.000-0.043, P<0.05) from 6 h to 7 d after exposure, and the contents of four kinds of amino acid neurotransmitter in hippocampus were decreased, of which GLU, GLY and GABA were decreased significantly (F=0.000-0.007, P<0.01) at 6 h after exposure, then tissue edema, neuronal degeneration, neuron mitochondria swelling and cavitation, endocytoplasmic reticulum broaden, synaptic cleft blurred, and perivascular space widen were found in the hippocampus at 6 h and 7 d after exposure. The changes in low dosage group were similar to those of the radiation group. However, in middle and high dosage groups, the abilities of learning and memory were normal to some extent with the significant differences compared to the radiation group from 6 h to 7 d after exposure(F=0.015-0.028, P<0.05). The contents of four kinds of amino acid neurotransmitter were not decreased, especially GLU contents close to normal level. There were significant differences between middle and high dosage groups and radiation group at 6 h after exposure (F=0.000-0.042, P<0.05). Moreover, no obvious injury in the hippocampus was observed in middle and high dosage groups at 6 h and 7 d after exposure. Conclusions Exposure to 30 mW/cm² microwave radiation could decrease the abilities of learning and memory, induce amino acid neurotransmitter turbulence, and injure the histology and ultrastructure of hippocampus. ADL at the dosages of 1.5 and 3 g ·kg^-1 ·d^-1 would have preventive effects on the injury induced by microwave exposure. The concentration of 1.5 g ·kg^-1 ·d^-1 of ADL might be the effective dosage to prevent the brain damage after microwave exposure.