萸连巴布剂的镇痛抗炎作用、刺激性及过敏性研究

目的:通过观察萸连巴布剂的镇痛抗炎作用、刺激性及过敏性,对萸连巴布剂的药效学及安全性进行评价,为萸连巴布剂的临床应用提供实验依据。方法:角叉菜胶致炎法致大鼠足肿胀;化学刺激法造小鼠镇痛抗炎模型;分别观察外用萸连巴布剂高、中、低3个剂量(含生药12,6,3 g.kg-1外用涂抹)组对足跖肿胀度、扭体次数及毛细血管通透性的影响。健康家兔分别单次和多次给药,进行完整与破损皮肤的皮肤刺激性试验;健康豚鼠,通过致敏接触和激发接触给药进行皮肤过敏试验。结果:萸连巴布剂中、低剂量组能明显降低大鼠足肿胀度,中剂量组作用时间持续时间较长。高、中剂量组降低毛细血管通透性,高、中、低3个剂量组能减少扭体次数,与赋形剂组比较均有显著性差异(P<0.05,P<0.01)。单次给药和连续给药,完整皮肤和破损皮肤组均未出现红斑、水肿等刺激反应。豚鼠皮肤未出现过敏反应。结论:萸连巴布剂具有镇痛抗炎和消肿的作用,对家兔完整皮肤、破损皮肤均无刺激性,对豚鼠皮肤不会引起过敏反应。

Study of Yulian Cataplasm on Analgesic, Anti-inflammatory Actions and Irritation, Hypersensitiveness

Objective: Analgesic,anti-inflammatory actions and irritation, hypersensitiveness were observed to evaluate the pharmacodynamics and safety of Yulian cataplasm, and to provide the experimental basis for clinical applications of the cataplasm. Method: The experiment animals were randomly divided into Yulian cataplasm high, middle and low dose groups(12,6,3 g·kg^-1).Carrageenan was used to induce rat paw edema. The mice inflammatory and algetic models were established by chemical irritation. Somatic writhing; foot swelling and capillary permeability were observed. The intact and damaged skins of rabbits were used to carry out irritation trial, and the hypersensitivity trial was conducted in guinea pigs. Result: Both middle and low dose groups of Yulian cataplasm could reduce rat paw swelling, middle dose group lasted longer duration of action. High and middle dose groups could decrease capillary permeability. All the three dose groups could reduce the writhing, and compared with excipient group (P<0.05, P<0.01). Irritation and hypersensitivity were not found. Conclusion: Yulian cataplasm could obviously exert both analgesic and anti-inflammatory actions, and no irritation and hypersensitivity were found.