天麻与钩藤配伍前后对SHR大鼠肾脏相关基因表达的影响

目的:研究天麻与钩藤配伍前后对SHR大鼠肾脏相关基因表达的影响。方法:设立空白、天麻混悬液(0.86 g.kg-1组)、钩藤煎液(1.17 g.kg-1组)、天麻钩藤合煎剂(天麻0.86 g.kg-1+钩藤1.17 g.kg-1)4个组别,连续ig给药10 d后提取肾脏mRNA,与基因芯片进行杂交,经扫描、计算机软件分析后观察配伍前后基因表达的变化。结果:筛选出天麻与钩藤配伍前后在肾脏中的目标基因(target gene)30个。结论:天麻配伍钩藤后,肾脏中调控血脂代谢、G-蛋白偶联受体介导的信号通路以及胰岛素抵抗相关基因的表达发生逆转,提示药对配伍后减弱了天麻对肾脏的相关作用。

Impact of Compatibility of Gastrodiae Rhizoma with Uncariae Ramulus Cum Uncis on Renal Gene Expression of Kidney in SHR Rats

Objective: To study the impact of compatibility of Gastrodiae Rhizoma with Uncariae Ramulus Cum Uncis on renal gene expression in SHR rats. Method: Four SHR rat groups were established, including Blank, Gastrodia suspension 0.86 g ·kg^-1 group, Uncariae Remulus Cum Unicis decoction 1.17 g ·kg^-1 group, Gastrodiae Rhizoma 0.8 g ·kg^-1 and Uncariae Ramulus Cum Uncis 1.17 g ·kg^-1 decoction combined. After 10 days of continuous oral administration, mRNA in the kidney of SHR rats was extracted, and then was hybrided with gene chip. After computerized scanning and analyzing, the changes in gene expression were observed. Result: Thirty target genes before and after compatibility of Gastrodiae Rhizoma with Uncariae Ramulus Cum Uncis were selected out. Conclution: After the compatibility of Gastrodiae Rhizoma with Uncariae Ramulus Cum Uncis, the trends of gene expression related with lipid metabolism, G-protein coupled receptor mediated signaling pathway, and insulin resistance are reversed. It indicates that the related effects of Gastrodiae Rhizoma on kidney are weakened after the compatibility.