至生胶囊调节食管癌造模大鼠细胞凋亡及Fas, FasL蛋白表达的研究

目的:观察至生胶囊对食管癌造模大鼠细胞凋亡及Fas,FasL蛋白表达的影响,从分子生物学角度分析其作用机制。方法:选用健康Wistar大鼠,除空白组外,其余大鼠均ip甲基戊基亚硝胺(MANA)5 mg.kg-1,1次/周,连续18周造模。末次注射后将造模大鼠随机分为模型组、至生胶囊高(0.75 g.kg-1)、中(0.5 g.kg-1)、低(0.25 g.kg-1)剂量组、西药替加氟组(20 mg.kg-1),ig,1次/d,连续4周。原位末端标记法(TUNEL)检测对食管癌癌细胞凋亡的促进作用;免疫组化法检测大鼠食管细胞中Fas,FasL蛋白的表达。结果:至生胶囊可明显促进食管癌细胞的凋亡,高、中、低剂量组、化疗组均促进凋亡,与模型组比较均有统计学意义(P<0.05),高、中剂量组疗效明显优于化疗组、低剂量组(P<0.05),且呈一定量效关系。各药物治疗组Fas表达阳性率均有不同程度的升高,FasL表达阳性率均有不同程度的降低。结论:至生胶囊能促进食管癌细胞凋亡,其作用机制可能与其提高Fas表达、降低FasL表达有关;至生胶囊促进食管癌细胞凋亡及调节Fas,FasL表达的作用存在一定的量效关系,至生胶囊高剂量此种作用优于替加氟。

Effect of Zhisheng Capsule on Cell Apoptosis and Fas,FasL Protein Expression in Rats Model of Esophageal Cancer

Objective: To observe the effect of Zhisheng Capsule on the rat model of esophageal cancer, inducing apoptosis and the impact on Fas, FasL protein expression. Method: Healthy Wistar rats were treated with MANA 5 mg·kg^-1 for 18 weeks except normal group.After the last injection,the rats were randomly divided into the following groups:esophageal model group, Zhisheng Capsule high(0.75 g·kg^-1), middle (0.5 g·kg^-1),low (0.25 g·kg^-1)dose groups, tegafur group (20 mg·kg^-1). TUNEL assay was used to examine the effect of Zhisheng Capsule on apoptosis of esophageal cancer cells. Immunohisto-chemistry (IHC) technique was used to measure the expression of Fas and FasL protein in cells. Result: Zhisheng Capsule of three doses could promote apoptosis of esophageal cancer cells according to the result of TUNEL assay. Comparison with model group was significant (P<0.05). High dose group was significantly better than chemotherapy group and low dose group (P<0.05). There showed some dose-effect relationship. Measured with IHC, the expression of Fas protein in the cells dealt with Zhisheng Capsule was increased, whereas the expression of FasL protein was decreased . Conclusion: Zhisheng Capsule could promote apoptosis of esophageal cancer cells. The molecular mechanism of Zhisheng Capsule may be related with up-regulating of Fas and down-regulating the expression of FasL. In addition, the effects of Zhisheng Capsule to promote apoptosis of esophageal cancer cells and Fas, FasL showed some dose-effect relationship. The effect of the capsule was better than tegafur.