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| 8-乙酰哈巴苷大鼠体内药代动力学 | |
| 引用本文： | 刘德文,张毅,李德凤,王锦玉,游云,曹岚,王芳,冯伟红,刘晓谦,陈两绵,仝燕.8-乙酰哈巴苷大鼠体内药代动力学[J].中国实验方剂学杂志,2011,17(20):129-132. |
| 作者姓名： | 刘德文 张毅 李德凤 王锦玉 游云 曹岚 王芳 冯伟红 刘晓谦 陈两绵 仝燕 |
| 作者单位： | 1. 中国中医科学院中药研究所,北京 100700;江西中医学院,南昌 330004 2. 中国中医科学院中药研究所,北京,100700 3. 江西中医学院,南昌,330004 |
| 基金项目： | "重大新药创制"科技重大专项 (2009ZX09301-005) |
| 摘 要： | 目的:建立血浆中8-乙酰哈巴苷的HPLC测定方法,研究大鼠不同给药途径给予8-乙酰哈巴苷后的药代动力学特征。方法:大鼠分别注射和灌胃8-乙酰哈巴苷,于不同时间点取血;采用HPLC,流动相乙腈-异丙醇-水(1.8∶10.2∶88),检测波长207 nm,流速1.0 mL.min-1,绘制药-时曲线,建立药代动力学模型,计算药代动力学参数。结果:8-乙酰哈巴苷在血浆中的回归方程为Y=613 753.13X-86 995.55(r=0.999 2),在0.015 3～22.995μg线性关系良好。高、中、低3个剂量组日内精密度RSD分别为0.59%,0.38%,0.55%;日间精密度RSD分别为3.9%,2.1%,1.5%。回收率分别为94.89%,95.85%,95.25%。灌胃途径在体内基本检测不出;注射途径3个剂量组主要药代动力学参数t1/2分别为(21.15±3.36),(24.26±5.50),(16.58±5.65)min;tmax分别为(4.98±0),(4.98±0),(4.98±0)min;Cmax分别为(2.25±1.41),(0.09±0.03),(0.02±0.007)g.L-1。结论:该法灵敏度高,专属性强,准确可靠,操作快速简便,可用于8-乙酰哈巴苷在大鼠体内的药代动力学研究,注射给予8-乙酰哈巴苷在大鼠体内吸收快且不完全,消除相对较慢,但具有良好的药代动力学特征,房室模型拟合分析为一房室模型。 |
| 关 键 词： | 8-乙酰哈巴苷 高效液相色谱法 不同给药途径 药时曲线 药代动力学 |
| 收稿时间： | 2011/6/17 0:00:00 |
Pharmacokinetic of 8-acetylharpagide in Rats |
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| LIU De-wen,ZHANG Yi,LI De-feng,WANG Jin-yu,YOU Yun,CAO Lan,WANG Fang,FENG Wei-hong,LIU Xiao-qian,CHEN Liang-mian and TONG Yan.Pharmacokinetic of 8-acetylharpagide in Rats[J].China Journal of Experimental Traditional Medical Formulae,2011,17(20):129-132. | |
| Authors: | LIU De-wen ZHANG Yi LI De-feng WANG Jin-yu YOU Yun CAO Lan WANG Fang FENG Wei-hong LIU Xiao-qian CHEN Liang-mian and TONG Yan |
| Institution: | Institute of Chinese Materia Medica, China Academy of Traditional Medical Sciences, Beijing 100700, China; Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China;Institute of Chinese Materia Medica, China Academy of Traditional Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Traditional Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Traditional Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Traditional Medical Sciences, Beijing 100700, China;Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China;Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China;Institute of Chinese Materia Medica, China Academy of Traditional Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Traditional Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Traditional Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Traditional Medical Sciences, Beijing 100700, China |
| Abstract: | |
| Keywords: | 8-acetylharpagide performance liquid chromatography different routes of administration concentration-time curve pharmacokinetics |
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