四逆散总生物碱分离纯化

目的:应用001×7阳离子树脂对四逆散中总生物碱成分进行分离纯化,并考察其纯化四逆散总生物碱的工艺参数。方法:考察洗脱溶剂、最佳上样浓度、吸附容量和洗脱溶剂用量等因素对阳离子树脂纯化四逆散总生物碱的影响。以辛弗林为对照品,用UV测定洗脱液中总生物碱的含量作为考察指标,确定最佳工艺参数。结果:洗脱溶剂应为4%氨水乙醇,最佳上样浓度为0.25 g.mL-1,吸附容量为5 mL,洗脱溶剂为8倍量吸附原液体积。结论:可以采用阳离子树脂纯化四逆散总生物碱,该纯化工艺简单、经济。

Separation and Purification process of Total Alkaloids from Sinisan

Objective: To study the purification technological parameters of total alkaloids from Sinisan by 001×7 cation resin. Method: Eluting solvent, sample concentration, absorption capacity and the volume of eluting solvent were chosen to analyze their influence on the purification process. UV was used to determine the process parameters by compared with synephrine, and the content of total alkaloids from eluent as criteria. Result: Eluting solvent was 4% ammonia ethanol solution, the optimum sample concentration was 0.25 g ·mL^-1, the absorption capacity is 5 mL and the volume of eluting solvent is eight times to the volume of sample solution. Conclusion: Total alkaloids from Sinisan can be purified by cation resins.The purification process is simple and affordable.