活血化瘀中药对脑出血大鼠脑组织 含水量及AQP4表达的影响

目的:观察活血化瘀药对脑出血大鼠脑组织含水量及AQP4表达的影响,以期从分子水平探讨其治疗出血性脑水肿的机制。方法:将80只雄性SD大鼠随机分为假手术组,模型组,活血高、低剂量组(10.4,2.6 g.kg-1),采用自体不凝血注入尾状核法制作大鼠脑出血模型,组织干湿重法计算脑含水量,免疫组织化学法和real-timePCR法检测脑组织AQP4蛋白和基因的表达。结果:模型组大鼠各时间点病灶侧脑含水量、血肿周围组织AQP4蛋白和基因的表达均明显高于假手术组(P<0.01);活血高、低剂量组大鼠病灶侧脑含水量及血肿周围组织AQP4的表达与同一时间点模型组相比均明显降低,活血高、低剂量组间也有统计学意义。结论:活血化瘀药能够减轻脑出血后脑水肿,其机制可能与降低AQP4表达有关。

Effect of Blood-activating and Stasis-removing Herbs on Brain Water Content and Aquaporin4 Expression in Intracerebral Hemorrhage Rats

Objective: To investigate the effects of blood-activating method on brain water content and aquaporin4(AQP4)expression in intracerebral hemorrhage(ICH)rats. Method: SD rats were randomly divided into the following groups:the sham operated group, model group, blood-activating high dose group and blood-activating low dose group. ICH rat model was established by injection autologous blood. The brain water content was determined by wet-dry weight,the expression of AQP4 protein and AQP4mRNA in cerebral tissues were determined respetively by immunohistochemitry techniques and real-time PCR. Result: Compared with those in the sham group, the expression of AQP4 mRNA and AQP4 protein increased significantly in the model group(P<0.01),and the brain water content showed the same trend. Compared with the model group, the expression of AQP4 mRNA and AQP4 protein and the brain water content decreased obviously in blood-activating groups(P<0.01), in a dose dependent manner. Conclusion: Blood-activating and stasis-removing herbs could decrease the edema of ICH, and the probable mechanism might be downregulation of AQP4.