开心解郁汤对抑郁模型大鼠脑内单胺类神经递质的影响

目的:观察开心解郁汤对抑郁模型大鼠脑内杏仁核去甲肾上腺素(NE)、多巴胺(DA)和5-羟色胺(5-HT)含量的影响。方法:将30只SD雄性大鼠随机分为正常组、模型组、给药组,每组10只;用慢性应激结合孤养的方式造模21 d。在造模同时,给药组ig开心解郁汤40 g.kg-1,1次/d,连续21 d。用高效液相-电化学方法检测大鼠脑内NE,DA,5-HT含量的变化。结果:与正常组比较,模型组大鼠脑内NE,DA,5-HT含量明显减少;与模型组比较,开心解郁汤可显著升高抑郁模型大鼠脑内NE,DA,5-HT的含量(P<0.05)。结论:开心解郁汤的抗抑郁作用机制可能与调节脑内单胺类神经递质的平衡有关。

Effect of Kaixin Jieyu Decoction on Concentraions of Monoamine Neurotransmitters in Brain of the Depression Rats

Objective: To investigate the effects of Kaixin Jieyu decoction on the concentraion changes of norepinephrine (NE),dopamine(DA) and serotomin(5-HT) in brain of depression model in rats. Method: Thirty male SD rats were evenly randomized into normal,model and medical groups. Depression model of rat was produced by using chronic unpredictable mild stress stimulation (forced ice-water swimming,electric shock,tail-clamping,etc) combined with lonely raising for 21 days.At the same time of producing the depression model,Kaixin Jieyu decoction (40 g ·kg^-1) was given to the medical group rats,once daily,continuously for 21 days.The concentraions of NE,DA and 5-HT were detected by HPLC-ECD. Result: The concentraions of NE,DA and 5-HT were reduced significantly in brain of the model rats compared to that of the normal group(P<0.05);also the concentraions of NE,DA and 5-HT were increased significantly in brain of Kaixin Jieyu decoction group compared to that of the model group (P<0.05). Conclusion: The mechanism for anti-depression of Kaixin Jieyu decoction may be correlative to regulating the balance of monamine transmitter.